ArticlePloS one2022
Genetic diversity and spatial distribution of Burkholderia mallei by core genome-based multilocus sequence typing analysis.
Article in PloS one, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 17 citations in OpenAlex.
- Virulome and phylogenomic profiling of a novel Burkholderia pseudomallei strain from an Indian clinical isolate.Molecular genetics and genomics : MGG · 2024Article
- BakRep - a searchable large-scale web repository for bacterial genomes, characterizations and metadata.Microbial genomics · 2024Article
- Development and implementation of a core genome multilocus sequence typing scheme forMicrobial genomics · 2024Article
- Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2024Article
- Assessment of the Virulence of theMicroorganisms · 2023Article
- Glanders Diagnosis in an Asymptomatic Mare from Brazil: Insights from Serology, Microbiological Culture, Mass Spectrometry, and Genome Sequencing.Pathogens (Basel, Switzerland) · 2023Article
- Molecular detection of Burkholderia mallei in different geographic regions of Brazil.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Article
- Influence of genomic variations on glanders serodiagnostic antigens using integrative genomic and transcriptomic approaches.Frontiers in veterinary science · 2023Article
- Virulence and resistance patterns ofFrontiers in microbiology · 2023Article
- Expression of virulence and antimicrobial related proteins in Burkholderia mallei and Burkholderia pseudomallei.PLoS neglected tropical diseases · 2023Article
- Sequence-based detection and typing procedures forFrontiers in veterinary science · 2022Review
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Burkholderia mallei is the etiological agent of glanders, a highly contagious and often fatal disease in equids. Due to the high genetic clonality of B. mallei, high-resolution typing assays are necessary to differentiate between individual strains. Here we report on the development and validation of a robust and reproducible core genome-based Multi Locus Sequence Typing Assay (cgMLST) for B. mallei, which is based on 3328 gene targets and enables high-resolution typing at the strain level. The assay was validated using a set of 120 B. mallei genomes from public databases and 23 newly sequenced outbreak strains from in-house strain collections. In this cgMLST analysis, strains from different geographic regions were clearly distinguished by at least 70 allele differences, allowing spatial clustering while closely related and epidemiologically related strains were separated by only zero to three alleles. Neither the different sequencing technologies nor the assembly strategies had an influence on the cgMLST results. The developed cgMLST is highly robust, reproducible and can be used for outbreak investigations, source tracking and molecular characterization of new B. mallei isolates.
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