ArticleNano letters2022
Single-Molecule Fluorescence Lifetime Imaging Using Wide-Field and Confocal-Laser Scanning Microscopy: A Comparative Analysis.
Article in Nano letters, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Self-Blinking Dye Restores Efficient Use of Nanobodies in Single-Molecule Localization Microscopy.Nano letters · 2026Article
- Emerging trends of fluorescence lifetime imaging microscopy (FLIM): advances, challenges, and prospects.Biophysics reports · 2026Article
- SIMBA: an agentic AI platform for single-molecule multidimensional imaging.bioRxiv : the preprint server for biology · 2026Article
- Two-dimensional fluorescence spectroscopy with entangled photons and time- and frequency-resolved two-photon coincidence detection.Science advances · 2026Article
- Spectroscopic DNA-PAINT for simultaneous multiplexed super-resolution microscopy.bioRxiv : the preprint server for biology · 2026Article
- Advances in fluorescence-based point-of-care diagnostics: probes, nanostructures and integrated systems.Journal of materials chemistry. C · 2026Review
- Intravital mesoscale optical imaging: challenges, techniques, and future perspectives.Biophysics reports · 2026Article
- Advancing Single-Molecule Biophysics: Next-Generation Organic Fluorophores with Tailored Labeling Strategies.Chemical & biomedical imaging · 2025Review
- Wide-field fluorescence lifetime imaging of single molecules with a gated single-photon camera.Light, science & applications · 2025Article
- Advanced fluorescence lifetime-enhanced multiplexed nanoscopy of cells.Methods in microscopy · 2025Article
- Spatiotemporal Exciton Tracking with a SPAD Camera.ACS photonics · 2025Article
- Super-resolution microscopy based on the inherent fluctuations of dye molecules.Biomedical optics express · 2025Article
- Single-molecule orientation-localization microscopy: Applications and approaches.Quarterly reviews of biophysics · 2024Review
- Towards measurements of absolute membrane potential in Bacillus subtilis using fluorescence lifetime.bioRxiv : the preprint server for biology · 2024Article
- Redefining Molecular Probes for Monitoring Subcellular Environment: A Perspective.Analytical chemistry · 2024Review
- Super-Resolution Microscopy as a Versatile Tool in Probing Molecular Assembly.International journal of molecular sciences · 2024Review
- Multiplexed Nanoscopy via Buffer Exchange.ACS nano · 2024Article
- A time-correlated single photon counting SPAD array camera with a bespoke data-processing algorithm for lightsheet fluorescence lifetime imaging (FLIM) and FLIM videos.Scientific reports · 2024Article
- Lipid-Based Nanotechnology: Liposome.Pharmaceutics · 2023Review
- Organic fluorescent probes for live-cell super-resolution imaging.Frontiers of optoelectronics · 2023Review
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A recent addition to the toolbox of super-resolution microscopy methods is fluorescence-lifetime single-molecule localization microscopy (FL-SMLM). The synergy of SMLM and fluorescence-lifetime imaging microscopy (FLIM) combines superior image resolution with lifetime information and can be realized using two complementary experimental approaches: confocal-laser scanning microscopy (CLSM) or wide-field microscopy. Here, we systematically and comprehensively compare these two novel FL-SMLM approaches in different spectral regions. For wide-field FL-SMLM, we use a commercial lifetime camera, and for CLSM-based FL-SMLM we employ a home-built system equipped with a rapid scan unit and a single-photon detector. We characterize the performances of the two systems in localizing single emitters in 3D by combining FL-SMLM with metal-induced energy transfer (MIET) for localization along the third dimension and in the lifetime-based multiplexed bioimaging using DNA-PAINT. Finally, we discuss advantages and disadvantages of wide-field and confocal FL-SMLM and provide practical advice on rational FL-SMLM experiment design.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.