Evidence map›Paper›PMID 35791599›Full record

ArticleSe pu = Chinese journal of chromatography2022

[In-site electrophoretic elution of excessive fluorescein isothiocyanate from fluorescent particles in gel for image analysis].

Guohong Chen, Zehua Guo, Yiren Cao, Liuyin Fan, Weiwen Liu, Yixin Ma, Chengxi Cao, Qiang Zhang

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Se pu = Chinese journal of chromatography, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 65% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. [Annual review of capillary electrophoresis technology in 2022].Se pu = Chinese journal of chromatography · 2023
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Guohong ChenSchool of Electronic Information & Electrical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.
Zehua GuoSchool of Electronic Information & Electrical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.
Yiren CaoSchool of Electronic Information & Electrical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.
Liuyin FanStudent Innovation Center, Shanghai Jiao Tong University, Shanghai 200240, China.
Weiwen LiuSchool of Electronic Information & Electrical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.
Yixin MaSchool of Electronic Information & Electrical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.
Chengxi CaoSchool of Electronic Information & Electrical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.
Qiang ZhangSchool of Electronic Information & Electrical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.
Shanghai Jiao Tong University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The sensitivity, accuracy, and efficiency of fluorescent particle detection can be improved by purifying the fluorescent-dye-labeled particles. In this study, an in-site model of electrophoretic elution (EE) was developed for the facile and efficient removal of unconjugated fluorescent dyes after labeling reactions, thereby facilitating the sensitive fluorescent imaging of proteins captured by microbeads. First, bovine serum albumin (BSA) and magnetic beads (MBs) were chosen as the model protein and particles, respectively, and an MBs-BSA complex was synthesized by mixing the beads with the BSA solution. Second, excessive fluorescein isothiocyanate (FITC) was added to the EP tube with MBs-BSA suspension for the fluorescent labeling of BSA, and a labeled compound was obtained after 8-h incubation in the dark at 4 ℃. The unpurified MBs-BSA

Indexed as

Fluorescent DyesSerum Albumin, BovineFluoresceinFluorescein-5-isothiocyanateHydrogelsFluoresceinFluorescein-5-isothiocyanateFluorescent DyesHydrogelsSerum Albumin, Bovineelectrophoresiselectrophoretic elutionfluorescent particlesimage detection

Identifiers

PMID35791599
PMCPMC9404076
OpenAlexW4284702611

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.