ArticleNature communications2022
Metaproteomics reveals enzymatic strategies deployed by anaerobic microbiomes to maintain lignocellulose deconstruction at high solids.
Article in Nature communications, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Diagnosing Anaerobic Digesters' Function and Performance: From Meta-Omics to Integrated Meta-Omics Analyses.Environmental microbiology reports · 2026Review
- Microbial consortia mediating lignocellulose turnover and denitrification in eutrophic lake sediment enrichments.mSystems · 2026Article
- Plant Biomass Depolymerization by Oxidoreductases: Structural Characteristics, Synergy Mechanism, and Evolutionary Adaptation.Journal of agricultural and food chemistry · 2026Review
- Comparative metagenomic and metatranscriptomic analyses reveal the role of the gayal rumen and hindgut microbiome in high-efficiency lignocellulose degradation.Journal of animal science and biotechnology · 2026Article
- A functional cascading of lignin modification via repression of caffeic acid O-methyltransferase for bioproduction and anti-oxidation in rice.Journal of advanced research · 2025Article
- Rhamnogalacturonan I is a recalcitrant pectin domain during Clostridium thermocellum-mediated deconstruction of switchgrass biomass.Biotechnology for biofuels and bioproducts · 2025Article
- Genomic and Functional Analysis of Auxiliary Activity Enzymes in the Maize Anthracnose PathogenMicroorganisms · 2025Article
- Overcoming Extreme Ammonia Inhibition on Methanogenesis by Artificially Constructing a Synergistically Community with Acidogenic Bacteria and Hydrogenotrophic Archaea.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- The microbiologist's guide to metaproteomics.iMeta · 2025Review
- TheProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Microbial consortia driving (ligno)cellulose transformation in agricultural woodchip bioreactors.Applied and environmental microbiology · 2024Article
- Microbial mechanisms for higher hydrogen production in anaerobic digestion at constant temperature versus gradient heating.Microbiome · 2024Article
- Microbial polyphenol metabolism is part of the thawing permafrost carbon cycle.Nature microbiology · 2024Article
- NovoLign: metaproteomics by sequence alignment.ISME communications · 2024Article
- metaSpectraST: an unsupervised and database-independent analysis workflow for metaproteomic MS/MS data using spectrum clustering.Microbiome · 2023Article
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Economically viable production of cellulosic biofuels requires operation at high solids loadings-on the order of 15 wt%. To this end we characterize Nature's ability to deconstruct and utilize mid-season switchgrass at increasing solid loadings using an anaerobic methanogenic microbiome. This community exhibits undiminished fractional carbohydrate solubilization at loadings ranging from 30 g/L to 150 g/L. Metaproteomic interrogation reveals marked increases in the abundance of specific carbohydrate-active enzyme classes. Significant enrichment of auxiliary activity family 6 enzymes at higher solids suggests a role for Fenton chemistry. Stress-response proteins accompanying these reactions are similarly upregulated at higher solids, as are β-glucosidases, xylosidases, carbohydrate-debranching, and pectin-acting enzymes-all of which indicate that removal of deconstruction inhibitors is important for observed undiminished solubilization. Our work provides insights into the mechanisms by which natural microbiomes effectively deconstruct and utilize lignocellulose at high solids loadings, informing the future development of defined cultures for efficient bioconversion.
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