ArticleNature communications2022
Healthy cloned offspring derived from freeze-dried somatic cells.
Article in Nature communications, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 34 citations in OpenAlex.
- Monosodium glutamate protects DNA of freeze-dried somatic cells and improves development of somatic cell nuclear transfer embryos.The Journal of reproduction and development · 2026Article
- Resurrection of chromosomes from frozen animals by single chromosome transfer into mouse oocytes.Scientific reports · 2026Article
- Limitations of serial cloning in mammals.Nature communications · 2026Article
- The challenges and prospects of mammalian reproduction in extraterrestrial environments.The Journal of reproduction and development · 2026Review
- Improved birth rates via rehydration of mouse freeze-dried spermatozoa using high-temperature ultrapure water.PloS one · 2026Article
- Freeze-Drying and Room Temperature Preservation of Mammalian Sperm.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Production of Cloned Bighorn Sheep Embryos Using ISCNT via HMC from Domestic Sheep Oocytes Treated with Resveratrol During IVM.Animals : an open access journal from MDPI · 2025Article
- Monosodium glutamate enhances freeze-dried mouse sperm quality during room-temperature storage.Scientific reports · 2025Article
- Can Humanity Thrive Beyond the Galaxy?The Journal of reproduction and development · 2025Review
- Method for long-term room temperature storage of mouse freeze-dried sperm.Scientific reports · 2025Article
- Production of Healthy Mice from Freeze-Dried Spermatozoa Preserved at Room Temperature.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Investigating cryopreservation techniques for maintaining morphology and in vitro viability of cartilage and skin from Spix's yellow-toothed cavies (Galea spixii Wagler, 1831) for conservation through biobanks.Journal of molecular histology · 2024Article
- Microwave-assisted dehydration, long-term storage at non-freezing temperatures, and rehydration of cat germinal vesicles†.Biology of reproduction · 2024Article
- A novel, simplified method to prepare and preserve freeze-dried mouse sperm in plastic microtubes.The Journal of reproduction and development · 2023Article
- Trehalose delivered by cold-responsive nanoparticles improves tolerance of cumulus-oocyte complexes to microwave drying.Journal of assisted reproduction and genetics · 2023Article
- Preparation and assessment of an optimized multichannel acellular nerve allograft for peripheral nerve regeneration.Bioengineering & translational medicine · 2023Article
- Mouse somatic cell nuclear transfer: What has changed and remained unchanged in 25 years.The Journal of reproduction and development · 2023Article
- Cloning by SCNT: Integrating Technical and Biology-Driven Advances.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Production of mouse offspring from zygotes fertilized with freeze-dried spermatids.Scientific reports · 2022Article
- Biomolecular Pathways of Cryoinjuries in Low-Temperature Storage for Mammalian Specimens.Bioengineering (Basel, Switzerland) · 2022Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Maintaining biodiversity is an essential task, but storing germ cells as genetic resources using liquid nitrogen is difficult, expensive, and easily disrupted during disasters. Our aim is to generate cloned mice from freeze-dried somatic cell nuclei, preserved at -30 °C for up to 9 months after freeze drying treatment. All somatic cells died after freeze drying, and nucleic DNA damage significantly increased. However, after nuclear transfer, we produced cloned blastocysts from freeze-dried somatic cells, and established nuclear transfer embryonic stem cell lines. Using these cells as nuclear donors for re-cloning, we obtained healthy cloned female and male mice with a success rate of 0.2-5.4%. Here, we show that freeze-dried somatic cells can produce healthy, fertile clones, suggesting that this technique may be important for the establishment of alternative, cheaper, and safer liquid nitrogen-free bio-banking solutions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.