ArticleNature communications2022
Elasticity of podosome actin networks produces nanonewton protrusive forces.
Article in Nature communications, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 32 citations in OpenAlex.
- Article
- ZO-1 shuttles between apical junctional complexes and podosomes by riding ERK activation waves.Nature communications · 2026Article
- 4polar3D single molecule imaging of 3D orientation in dense actin networks using ratiometric polarization splitting.Nature communications · 2026Article
- Podosomes and Mechanical Force.Results and problems in cell differentiation · 2026Review
- Myosins 1e/f at the podosome base regulate podosome dynamics and promote macrophage migration.European journal of cell biology · 2025Article
- Giant activity-induced elasticity in entangled polymer solutions.Nature communications · 2025Article
- Dynamic clamping induces rotation-to-beating transition of pinned filaments in gliding assays.Journal of the Royal Society, Interface · 2025Article
- The Molecular Mechanism of Macrophages in Response to Mechanical Stress.Annals of biomedical engineering · 2025Review
- Myosin forces elicit an F-actin structural landscape that mediates mechanosensitive protein recognition.bioRxiv : the preprint server for biology · 2024Article
- The Interaction of Mechanics and the Hippo Pathway inCancers · 2023Article
- Chemo-mechanical diffusion waves explain collective dynamics of immune cell podosomes.Nature communications · 2023Article
- Actin Bundles Dynamics and Architecture.Biomolecules · 2023Review
- Deciphering the molecular mechanisms of actin cytoskeleton regulation in cell migration using cryo-EM.Biochemical Society transactions · 2023Review
- Article
- Actin nano-architecture of phagocytic podosomes.Nature communications · 2022Article
- Elasticity of podosome actin networks produces nanonewton protrusive forces.Nature communications · 2022Article
- Nanoscale architecture and coordination of actin cores within the sealing zone of human osteoclasts.eLife · 2022Article
- Appreciating the role of cell shape changes in the mechanobiology of epithelial tissues.Biophysics reviews · 2022Review
Corrections and comments
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Authors and funding
12 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Actin filaments assemble into force-generating systems involved in diverse cellular functions, including cell motility, adhesion, contractility and division. It remains unclear how networks of actin filaments, which individually generate piconewton forces, can produce forces reaching tens of nanonewtons. Here we use in situ cryo-electron tomography to unveil how the nanoscale architecture of macrophage podosomes enables basal membrane protrusion. We show that the sum of the actin polymerization forces at the membrane is not sufficient to explain podosome protrusive forces. Quantitative analysis of podosome organization demonstrates that the core is composed of a dense network of bent actin filaments storing elastic energy. Theoretical modelling of the network as a spring-loaded elastic material reveals that it exerts forces of a few tens of nanonewtons, in a range similar to that evaluated experimentally. Thus, taking into account not only the interface with the membrane but also the bulk of the network, is crucial to understand force generation by actin machineries. Our integrative approach sheds light on the elastic behavior of dense actin networks and opens new avenues to understand force production inside cells.
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