Evidence map›Paper›PMID 35788779›Full record

ArticleProtoplasma2023

Physiological, epigenetic, and proteomic responses in Pfaffia glomerata growth in vitro under salt stress and 5-azacytidine.

Evandro Alexandre Fortini, Diego Silva Batista, Sérgio Heitor Sousa Felipe, Tatiane Dulcineia Silva, Ludmila Nayara Freitas Correia, Letícia Monteiro Farias, Daniele Vidal Faria, Vitor Batista Pinto, Claudete Santa-Catarina, Vanildo Silveira and 3 more

Abstract read
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In one paragraph

Article in Protoplasma, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.8field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 12 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Review
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 6 institutions in 2 countries.

Evandro Alexandre FortiniLaboratório de Cultura de Tecidos Vegetais (LCTII), Departamento de Biologia Vegetal/BIOAGRO, Universidade Federal de Viçosa, Campus Universitário, Avenida Peter Henry Rolfs s/n, Viçosa, MG, 36570-900, Brazil.ORCID http://orcid.org/0000-0002-4127-8872
Diego Silva BatistaDepartamento de Agricultura, Universidade Federal da Paraíba, Campus III, Bananeiras, PB, 58220-000, Brazil.ORCID http://orcid.org/0000-0002-5798-7761
Sérgio Heitor Sousa FelipePPG em Agroecologia, Universidade Estadual do Maranhão, Av. Lourenço Vieira da Silva, s/nº, Cidade Universitária Paulo VI, São Luís, MA, Brazil.ORCID http://orcid.org/0000-0003-4529-9547
Tatiane Dulcineia SilvaLaboratório de Cultura de Tecidos Vegetais (LCTII), Departamento de Biologia Vegetal/BIOAGRO, Universidade Federal de Viçosa, Campus Universitário, Avenida Peter Henry Rolfs s/n, Viçosa, MG, 36570-900, Brazil.ORCID http://orcid.org/0000-0003-2253-3847
Ludmila Nayara Freitas CorreiaLaboratório de Cultura de Tecidos Vegetais (LCTII), Departamento de Biologia Vegetal/BIOAGRO, Universidade Federal de Viçosa, Campus Universitário, Avenida Peter Henry Rolfs s/n, Viçosa, MG, 36570-900, Brazil.ORCID http://orcid.org/0000-0002-7968-0795
Letícia Monteiro FariasDepartamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Viçosa, MG, 36570-900, Brazil.ORCID http://orcid.org/0000-0003-3176-564X
Daniele Vidal FariaLaboratório de Cultura de Tecidos Vegetais (LCTII), Departamento de Biologia Vegetal/BIOAGRO, Universidade Federal de Viçosa, Campus Universitário, Avenida Peter Henry Rolfs s/n, Viçosa, MG, 36570-900, Brazil.ORCID http://orcid.org/0000-0001-9622-8074
Vitor Batista PintoLaboratório de Biotecnologia (LBT), Centro de Biociências e Biotecnologia (CBB), Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Av. Alberto Lamego 2000, Campos dos Goytacazes, RJ, 28013-602, Brazil.ORCID http://orcid.org/0000-0002-8119-7268
Claudete Santa-CatarinaLaboratório de Biologia Celular e Tecidual (LBCT), CBB-UENF, Campos dos Goytacazes, RJ, Brazil.ORCID http://orcid.org/0000-0002-1669-660X
Vanildo SilveiraLaboratório de Biotecnologia (LBT), Centro de Biociências e Biotecnologia (CBB), Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Av. Alberto Lamego 2000, Campos dos Goytacazes, RJ, 28013-602, Brazil.ORCID http://orcid.org/0000-0001-5697-6932
Clelia De-la-PeñaUnidad de Biotecnología, Centro de Investigación Científica de Yucatán, A. C. (CICY), 97205, Mérida, Yucatán, Mexico.ORCID http://orcid.org/0000-0002-9093-9489
Eduardo Castillo-CastroUnidad de Biotecnología, Centro de Investigación Científica de Yucatán, A. C. (CICY), 97205, Mérida, Yucatán, Mexico.
Wagner Campos OtoniLaboratório de Cultura de Tecidos Vegetais (LCTII), Departamento de Biologia Vegetal/BIOAGRO, Universidade Federal de Viçosa, Campus Universitário, Avenida Peter Henry Rolfs s/n, Viçosa, MG, 36570-900, Brazil. wcotoni@gmail.com.ORCID http://orcid.org/0000-0002-9614-9373
Universidade Federal de Viçosa · BRSecretaría de Investigación, Innovación y Educación Superior · MXState University of Norte Fluminense · BRPrefeitura Municipal de Campos dos Goytacazes · BRUniversidade Estadual do Maranhão · BRUniversidade Federal da Paraíba · BR

Funding

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior Finance Code 001
6 · The paper itself

Abstract

Plants adjust their complex molecular, biochemical, and metabolic processes to overcome salt stress. Here, we investigated the proteomic and epigenetic alterations involved in the morphophysiological responses of Pfaffia glomerata, a medicinal plant, to salt stress and the demethylating agent 5-azacytidine (5-azaC). Moreover, we investigated how these changes affected the biosynthesis of 20-hydroxyecdysone (20-E), a pharmacologically important specialized metabolite. Plants were cultivated in vitro for 40 days in Murashige and Skoog medium supplemented with NaCl (50 mM), 5-azaC (25 μM), and NaCl + 5-azaC. Compared with the control (medium only), the treatments reduced growth, photosynthetic rates, and photosynthetic pigment content, with increase in sucrose, total amino acids, and proline contents, but a reduction in starch and protein. Comparative proteomic analysis revealed 282 common differentially accumulated proteins involved in 87 metabolic pathways, most of them related to amino acid and carbohydrate metabolism, and specialized metabolism. 5-azaC and NaCl + 5-azaC lowered global DNA methylation levels and 20-E content, suggesting that 20-E biosynthesis may be regulated by epigenetic mechanisms. Moreover, downregulation of a key protein in jasmonate biosynthesis indicates the fundamental role of this hormone in the 20-E biosynthesis. Taken together, our results highlight possible regulatory proteins and epigenetic changes related to salt stress tolerance and 20-E biosynthesis in P. glomerata, paving the way for future studies of the mechanisms involved in this regulation.

Indexed as

AmaranthaceaeProteomicsAzacitidineEpigenesis, GeneticPlant ProteinsSalt ToleranceSodium ChlorideStress, PhysiologicalAzacitidinePlant ProteinsSodium ChlorideAbiotic stressDNA methylationMedicinal plantMethyltransferase inhibitorPhytoecdysteroidsProteomics

Identifiers

PMID35788779
OpenAlexW4283806169

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.