Evidence map›Paper›PMID 35788760›Full record

ArticleNature metabolism2022

Targeted erasure of DNA methylation by TET3 drives adipogenic reprogramming and differentiation.

Jeu Park, Do Hoon Lee, Seokjin Ham, Jiyoung Oh, Jung-Ran Noh, Yun Kyung Lee, Yoon Jeong Park, Gung Lee, Sang Mun Han, Ji Seul Han and 11 more

Abstract read
PubMed Publisher
In one paragraph

Article in Nature metabolism, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
2.3field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 30 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

21 authors at 5 institutions in 2 countries.

Jeu ParkCenter for Adipocyte Structure and Function, Institute of Molecular Biology and Genetics, School of Biological Sciences, Seoul National University, Seoul, South Korea.
Do Hoon LeeBioinformatics Institute, Seoul National University, Seoul, South Korea.ORCID http://orcid.org/0000-0003-2163-5489
Seokjin HamDepartment of Life Sciences, Pohang University of Science and Technology, Pohang, South Korea.
Jiyoung OhDepartment of Biological Sciences, College of Information and Bioengineering, Ulsan National Institute of Science and Technology, Ulsan, South Korea.
Jung-Ran NohLaboratory Animal Resource Center, Korea Research Institute of Bioscience and Biotechnology, University of Science and Technology, Daejeon, South Korea.
Yun Kyung LeeInternal Medicine, Seoul National University College of Medicine & Seoul National University Bundang Hospital, Seoul, South Korea.
Yoon Jeong ParkCenter for Adipocyte Structure and Function, Institute of Molecular Biology and Genetics, School of Biological Sciences, Seoul National University, Seoul, South Korea.
Gung LeeCenter for Adipocyte Structure and Function, Institute of Molecular Biology and Genetics, School of Biological Sciences, Seoul National University, Seoul, South Korea.
Sang Mun HanCenter for Adipocyte Structure and Function, Institute of Molecular Biology and Genetics, School of Biological Sciences, Seoul National University, Seoul, South Korea.
Ji Seul HanCenter for Adipocyte Structure and Function, Institute of Molecular Biology and Genetics, School of Biological Sciences, Seoul National University, Seoul, South Korea.ORCID http://orcid.org/0000-0003-1645-2952
Ye Young KimCenter for Adipocyte Structure and Function, Institute of Molecular Biology and Genetics, School of Biological Sciences, Seoul National University, Seoul, South Korea.ORCID http://orcid.org/0000-0002-3598-5757
Yong Geun JeonCenter for Adipocyte Structure and Function, Institute of Molecular Biology and Genetics, School of Biological Sciences, Seoul National University, Seoul, South Korea.
Han NahmgoongCenter for Adipocyte Structure and Function, Institute of Molecular Biology and Genetics, School of Biological Sciences, Seoul National University, Seoul, South Korea.
Kyung Cheul ShinCenter for Adipocyte Structure and Function, Institute of Molecular Biology and Genetics, School of Biological Sciences, Seoul National University, Seoul, South Korea.
Sung Min KimCenter for Adipocyte Structure and Function, Institute of Molecular Biology and Genetics, School of Biological Sciences, Seoul National University, Seoul, South Korea.
Sung Hee ChoiInternal Medicine, Seoul National University College of Medicine & Seoul National University Bundang Hospital, Seoul, South Korea.
Chul-Ho LeeLaboratory Animal Resource Center, Korea Research Institute of Bioscience and Biotechnology, University of Science and Technology, Daejeon, South Korea.ORCID http://orcid.org/0000-0002-6996-5746
Jiyoung ParkDepartment of Biological Sciences, College of Information and Bioengineering, Ulsan National Institute of Science and Technology, Ulsan, South Korea.ORCID http://orcid.org/0000-0003-3705-4769
Tae Young RohDepartment of Life Sciences, Pohang University of Science and Technology, Pohang, South Korea.ORCID http://orcid.org/0000-0001-5833-0844
Sun KimDepartment of Computer Science and Engineering, Institute of Engineering Research, Seoul National University, Seoul, South Korea.
Jae Bum KimCenter for Adipocyte Structure and Function, Institute of Molecular Biology and Genetics, School of Biological Sciences, Seoul National University, Seoul, South Korea. jaebkim@snu.ac.kr.ORCID http://orcid.org/0000-0003-2337-6935
Seoul National University · KRKorea Research Institute of Bioscience and Biotechnology · KRPohang University of Science and Technology · KRSeoul National University Bundang Hospital · KRUlsan National Institute of Science and Technology · KR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

DNA methylation is a crucial epigenetic modification in the establishment of cell-type-specific characteristics. However, how DNA methylation is selectively reprogrammed at adipocyte-specific loci during adipogenesis remains unclear. Here, we show that the transcription factor, C/EBPδ, and the DNA methylation eraser, TET3, cooperatively control adipocyte differentiation. We perform whole-genome bisulfite sequencing to explore the dynamics and regulatory mechanisms of DNA methylation in adipocyte differentiation. During adipogenesis, DNA methylation selectively decreases at adipocyte-specific loci carrying the C/EBP binding motif, which correlates with the activity of adipogenic promoters and enhancers. Mechanistically, we find that C/EBPδ recruits a DNA methylation eraser, TET3, to catalyse DNA demethylation at the C/EBP binding motif and stimulate the expression of key adipogenic genes. Ectopic expression of TET3 potentiates in vitro and in vivo adipocyte differentiation and recovers downregulated adipogenic potential, which is observed in aged mice and humans. Taken together, our study highlights how targeted reprogramming of DNA methylation through cooperative action of the transcription factor C/EBPδ, and the DNA methylation eraser TET3, controls adipocyte differentiation.

Indexed as

AdipogenesisDioxygenasesAnimalsCCAAT-Enhancer-Binding ProteinsCell DifferentiationDNA MethylationEpigenesis, GeneticHumansMiceTranscription FactorsCCAAT-Enhancer-Binding ProteinsC-EPB transcription factorDioxygenasesTET3 protein, humanTet3 protein, mouseTranscription Factors

Identifiers

PMID35788760
OpenAlexW4283803435

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.