ArticlePLoS genetics2022
Base editing in bovine embryos reveals a species-specific role of SOX2 in regulation of pluripotency.
Article in PLoS genetics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 39 citations in OpenAlex.
- Molecular Regulation of Post-Hatching Embryo Development: Implications for Early Pregnancy Losses in Livestock Species.Reproduction in domestic animals = Zuchthygiene · 2026Review
- Genomic evaluation and gene expression analysis of preimplantation embryos for enhancing cattle breeding and in vitro embryo production.The Journal of reproduction and development · 2026Review
- Species-specific roles of SP1 in bovine and human embryonic genome activation and early embryonic development.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Effects of leonurine supplementation during in vitro culture on oxidative stress, cell proliferation, apoptosis, and autophagy in bovine embryos.Scientific reports · 2026Article
- Role of Glutathione in Alleviating Chilling Injury in Bovine Blastocysts: Mitochondrial Restoration and Apoptosis Inhibition.Antioxidants (Basel, Switzerland) · 2026Article
- Analysis of Cytosine Base Editors in Bovine Zygotes: Efficiency and Editing Window Characterization Through Targeting theCurrent issues in molecular biology · 2025Article
- Effect of Bovine Follicular Fluid Small Extracellular Vesicles Isolated by Ultracentrifugation and Chromatography on In Vitro Oocyte Maturation and Embryo Development.International journal of molecular sciences · 2025Article
- An advanced cytosine base editor enabled the generation of cattle with a stop codon in the β-lactoglobulin gene.Transgenic research · 2025Article
- Delayed Blastocyst Formation Reduces the Quality and Hatching Ability of Porcine Parthenogenetic Blastocysts by Increasing DNA Damage, Decreasing Cell Proliferation, and Altering Transcription Factor Expression Patterns.Journal of developmental biology · 2024Article
- Evaluation of bovine embryo quality based on gene expression profiling using whole-transcriptome amplification.The Journal of reproduction and development · 2024Article
- Pre-Implantation Bovine Embryo Evaluation-From Optics to Omics and Beyond.Animals : an open access journal from MDPI · 2023Review
- Bovine blastocyst-like structures derived from stem cell cultures.Cell stem cell · 2023Article
- A conserved role of the Hippo signalling pathway in initiation of the first lineage specification event across mammals.Development (Cambridge, England) · 2023Article
- Functional role of GATA3 and CDX2 in lineage specification during bovine early embryonic development.Reproduction (Cambridge, England) · 2023Article
- Preimplantation Developmental Competence of Bovine and Porcine Oocytes Activated by Zinc Chelation.Animals : an open access journal from MDPI · 2022Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The emergence of the first three lineages during development is orchestrated by a network of transcription factors, which are best characterized in mice. However, the role and regulation of these factors are not completely conserved in other mammals, including human and cattle. Here, we establish a gene inactivation system with a robust efficiency by introducing premature codon with cytosine base editors in bovine early embryos. By using this approach, we have determined the functional consequences of three critical lineage-specific genes (SOX2, OCT4 and CDX2) in bovine embryos. In particular, SOX2 knockout results in a failure of the establishment of pluripotency in blastocysts. Indeed, OCT4 level is significantly reduced and NANOG barely detectable. Furthermore, the formation of primitive endoderm is compromised with few SOX17 positive cells. RNA-seq analysis of single blastocysts (day 7.5) reveals dysregulation of 2074 genes, among which 90% are up-regulated in SOX2-null blastocysts. Intriguingly, more than a dozen lineage-specific genes, including OCT4 and NANOG, are down-regulated. Moreover, SOX2 level is sustained in the trophectoderm in absence of CDX2. However, OCT4 knockout does not affect the expression of SOX2. Overall, we propose that SOX2 is indispensable for OCT4 and NANOG expression and CDX2 represses the expression of SOX2 in the trophectoderm in cattle, which are all in sharp contrast with results in mice.
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