ArticleAnalytical chemistry2022
High Resolution Ambient MS Imaging of Biological Samples by Desorption Electro-Flow Focussing Ionization.
Article in Analytical chemistry, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Single-cell mass spectrometry imaging: platform advances for multimodal spatial omics.Analytical and bioanalytical chemistry · 2026Review
- Targeting the Lipid Metabolism Proteins FASN and GPAM in Alveolar Type II Cells Decreases Lung Metastasis.Cancer discovery · 2026Article
- Early-stage drug discovery in a new-generation ultrahigh-throughput mass spectrometry platform.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Ambient high-spatial-resolution mass spectrometry imaging: recent advances in technologies and metabolomics studies.Trends in analytical chemistry : TRAC · 2026Article
- Comparison of Mass Spectrometry Imaging by Desorption Electrospray Ionization (DESI) and Desorption Electro-Flow Focusing Ionization (DEFFI).Metabolites · 2026Article
- Topological segmentation of mass spectrometry imaging data.Journal of mass spectrometry and advances in the clinical lab · 2025Article
- Subcellular-Resolution Molecular Pathology by Laser Ablation-Rapid Evaporative Ionization Mass Spectrometry.Analytical chemistry · 2025Article
- Mass Spectrometry Imaging.Analytical chemistry · 2025Review
- Single-cell mass spectrometry imaging of lipids in HeLa cells via tapping-mode scanning probe electrospray ionization.Communications chemistry · 2025Article
- Delineation of Subcellular Molecular Heterogeneity in Single Cells via Ultralow-Flow-Rate Desorption Electrospray Ionization Mass Spectrometry Imaging.Analytical chemistry · 2025Article
- Improvements in Fast Mass Microscopy for Large-Area Samples.Analytical chemistry · 2024Article
- Improved Detection of Tryptic Peptides from Tissue Sections Using Desorption Electrospray Ionization Mass Spectrometry Imaging.Journal of the American Society for Mass Spectrometry · 2024Article
- Spatial pharmacology using mass spectrometry imaging.Trends in pharmacological sciences · 2024Review
- Vitamin BNature metabolism · 2023Article
- A monolithic microfluidic probe for ambient mass spectrometry imaging of biological tissues.Lab on a chip · 2023Article
- High-resolution integrated microfluidic probe for mass spectrometry imaging of biological tissues.Analytica chimica acta · 2023Article
- Promise of spatially resolved omics for tumor research.Journal of pharmaceutical analysis · 2023Review
- Applications of ambient ionization mass spectrometry in 2022: An annual review.Analytical science advances · 2023Review
- Article
- A mass spectrometry imaging approach on spatiotemporal distribution of multiple alkaloids inFrontiers in plant science · 2022Article
Corrections and comments
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Authors and funding
14 authors.
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Abstract
In this study, we examine the suitability of desorption electro-flow focusing ionization (DEFFI) for mass spectrometry imaging (MSI) of biological tissue. We also compare the performance of desorption electrospray ionization (DESI) with and without the flow focusing setup. The main potential advantages of applying the flow focusing mechanism in DESI is its rotationally symmetric electrospray jet, higher intensity, more controllable parameters, and better portability due to the robustness of the sprayer. The parameters for DEFFI have therefore been thoroughly optimized, primarily for spatial resolution but also for intensity. Once the parameters have been optimized, DEFFI produces similar images to the existing DESI. MS images for mouse brain samples, acquired at a nominal pixel size of 50 μm, are comparable for both DESI setups, albeit the new sprayer design yields better sensitivity. Furthermore, the two methods are compared with regard to spectral intensity as well as the area of the desorbed crater on rhodamine-coated slides. Overall, the implementation of a flow focusing mechanism in DESI is shown to be highly suitable for imaging biological tissue and has potential to overcome some of the shortcomings experienced with the current geometrical design of DESI.
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