ArticleCell reports methods2022
A versatile viral toolkit for functional discovery in the nervous system.
Article in Cell reports methods, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- A Cre-dependent lentiviral vector for neuron subtype-specific expression of large proteins.FEBS letters · 2026Article
- Neuropeptides as transmitters and regulators of the locus coeruleus noradrenergic system.Pharmacological reviews · 2025Review
- Neurogliaform Cells Exhibit Laminar-specific Responses in the Visual Cortex and Modulate Behavioral State-dependent Cortical Activity.Research square · 2024Article
- A single-vector intersectional AAV strategy for interrogating cellular diversity and brain function.Nature neuroscience · 2024Article
- Metabotropic signaling within somatostatin interneurons controls transient thalamocortical inputs during development.Nature communications · 2024Article
- Neurogliaform Cells Exhibit Laminar-specific Responses in the Visual Cortex and Modulate Behavioral State-dependent Cortical Activity.bioRxiv : the preprint server for biology · 2024Article
- Functional specialization of hippocampal somatostatin-expressing interneurons.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Sex diversity in the 21st century: Concepts, frameworks, and approaches for the future of neuroendocrinology.Hormones and behavior · 2024Article
- Physiological features of parvalbumin-expressing GABAergic interneurons contributing to high-frequency oscillations in the cerebral cortex.Current research in neurobiology · 2024Review
- Genetic approaches to elucidating cortical and hippocampal GABAergic interneuron diversity.Frontiers in cellular neuroscience · 2024Review
- Article
- Optical opening of the blood-brain barrier for targeted and ultra-sparse viral infection of cells in mouse cortex.Cell reports methods · 2023Article
- Functional specialization of hippocampal somatostatin-expressing interneurons.bioRxiv : the preprint server for biology · 2023Article
- Advances in AAV technology for delivering genetically encoded cargo to the nonhuman primate nervous system.Current research in neurobiology · 2023Review
- Advances in approaches to study cell-type specific cortical circuits throughout development.Frontiers in cellular neuroscience · 2022Review
Corrections and comments
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Authors and funding
20 authors.
Funding
Abstract
The ability to precisely control transgene expression is essential for basic research and clinical applications. Adeno-associated viruses (AAVs) are non-pathogenic and can be used to drive stable expression in virtually any tissue, cell type, or species, but their limited genomic payload results in a trade-off between the transgenes that can be incorporated and the complexity of the regulatory elements controlling their expression. Resolving these competing imperatives in complex experiments inevitably results in compromises. Here, we assemble an optimized viral toolkit (VTK) that addresses these limitations and allows for efficient combinatorial targeting of cell types. Moreover, their modular design explicitly enables further refinements. We achieve this in compact vectors by integrating structural improvements of AAV vectors with innovative molecular tools. We illustrate the potential of this approach through a systematic demonstration of their utility for targeting cell types and querying their biology using a wide array of genetically encoded tools.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.