ArticleBiomedical optics express2022
SOLEIL: single-objective lens inclined light sheet localization microscopy.
Article in Biomedical optics express, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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The trial behind it
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Who cites it
9 citing papers in PubMed.
- High-speed volumetric single-molecule imaging using dual-wavelength light sheets and PSF-engineered enhanced biplane detection.bioRxiv : the preprint server for biology · 2026Article
- Oblique line scan illumination enables expansive, accurate and sensitive single-protein measurements in solution and in living cells.Nature methods · 2025Article
- Whole-cell multi-target single-molecule super-resolution imaging in 3D with microfluidics and a single-objective tilted light sheet.Nature communications · 2024Article
- Whole-cell multi-target single-molecule super-resolution imaging in 3D with microfluidics and a single-objective tilted light sheet.bioRxiv : the preprint server for biology · 2024Article
- Multimodal illumination platform for 3D single-molecule super-resolution imaging throughout mammalian cells.Biomedical optics express · 2024Article
- Full-aperture extended-depth oblique plane microscopy through dynamic remote focusing.Journal of biomedical optics · 2024Article
- Light sheet illumination in single-molecule localization microscopy for imaging of cellular architectures and molecular dynamics.Npj imaging · 2024Review
- Editorial: Adaptive optics forFrontiers in neuroscience · 2023Article
- Adaptive optics in single objective inclined light sheet microscopy enables three-dimensional localization microscopy in adultFrontiers in neuroscience · 2022Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
High-NA light sheet illumination can improve the resolution of single-molecule localization microscopy (SMLM) by reducing the background fluorescence. These approaches currently require custom-made sample holders or additional specialized objectives, which makes the sample mounting or the optical system complex and therefore reduces the usability of these approaches. Here, we developed a single-objective lens-inclined light sheet microscope (SOLEIL) that is capable of 2D and 3D SMLM in thick samples. SOLEIL combines oblique illumination with point spread function PSF engineering to enable dSTORM imaging in a wide variety of samples. SOLEIL is compatible with standard sample holders and off-the-shelve optics and standard high NA objectives. To accomplish optimal optical sectioning we show that there is an ideal oblique angle and sheet thickness. Furthermore, to show what optical sectioning delivers for SMLM we benchmark SOLEIL against widefield and HILO microscopy with several biological samples. SOLEIL delivers in 15
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.