Evidence map›Paper›PMID 35781204›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Plasmid-Based Gene Knockout Strategy with Subsequent Marker Recycling in Pichia pastoris.

Simon Kobalter, Astrid Radkohl, Helmut Schwab, Anita Emmerstorfer-Augustin, Harald Pichler

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Current advances ofFrontiers in microbiology · 2022
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Simon KobalterGraz Institute of Molecular Biotechnology, University of Technology, Graz, Austria.
Astrid RadkohlGraz Institute of Molecular Biotechnology, University of Technology, Graz, Austria.
Helmut SchwabGraz Institute of Molecular Biotechnology, University of Technology, Graz, Austria.
Anita Emmerstorfer-AugustinGraz Institute of Molecular Biotechnology, University of Technology, Graz, Austria.
Harald PichlerGraz Institute of Molecular Biotechnology, University of Technology, Graz, Austria. harald.pichler@tugraz.at.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Gene knockout is a key technology in the development of cell factories and basic research alike. The methylotrophic yeast Pichia pastoris is typically employed as a producer of proteins and of fine chemicals, due to its ability to accumulate high cell densities in conjunction with a set of strong inducible promoters. However, protocols for genome engineering in this host are still cumbersome and time-consuming. Moreover, extensive genome engineering raises the need for a multitude of selection markers, which are limited in P. pastoris. In this chapter, we describe a fast and efficient method for gene disruption in P. pastoris that utilizes marker recycling to enable repetitive genome engineering cycles. A set of ready-to-use knockout vectors simplifies cloning procedures and facilitates quick knockout generation.

Indexed as

SaccharomycetalesBiomarkersGene Knockout TechniquesPlasmidsSaccharomyces cerevisiaeBiomarkersGene disruptionHomologous recombinationKnockout plasmidsMarker recyclingPichia pastoris

Identifiers

PMID35781204

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.