ArticleMethods in molecular biology (Clifton, N.J.)2022
Competent Cell Preparation and Transformation of Pichia pastoris.
Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed.
- Heterologous expression of hagfish thread proteins EsTKα and EsTKγ inSynthetic and systems biotechnology · 2026Article
- A Rabies Virus Glycoprotein Subunit Vaccine Produced inVaccines · 2026Article
- Gastric-protective and antioxidant effects of fermentedOpen life sciences · 2026Article
- A Split-Marker System for CRISPR-Cas9 Genome Editing in Methylotrophic Yeasts.International journal of molecular sciences · 2023Article
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Authors and funding
3 authors.
Funding
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Abstract
During the past three decades, the methylotrophic yeast Pichia pastoris (recently reclassified as Komagataella phaffii) has gained widespread acceptance as a system of choice for heterologous protein expression. One of the reasons that this yeast is used so frequently is the simplicity of techniques required for its molecular genetic manipulation. There are several different protocols available for introducing DNA into P. pastoris using electroporation or heat shock. We describe here a shortened protocol for cell preparation and transformation that works reliably with either prototrophic markers or antibiotic selection in this host. This procedure utilizes the most efficient portions of the electroporation and heat-shock transformation protocols to yield a method that is both time-saving and effective.
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Registered trials
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