ArticlePlant biotechnology journal2022
Editing melon eIF4E associates with virus resistance and male sterility.
Article in Plant biotechnology journal, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 52 citations in OpenAlex.
- Conserved Arginine of the Potyviridae Viral Genome-Linked Proteins (VPg) as a Key Determinant for eIF4E Binding.International journal of molecular sciences · 2026Article
- Functional Validation of the Melon Fom-1 Gene, Controlling Resistance to Fusarium oxysporum Races 0 and 2, by CRISPR/Cas9 Mutagenesis.Molecular plant pathology · 2026Article
- Molecular gatekeepers: eukaryotic translation factors decoding plant-virus dynamics for resistance engineering.Stress biology · 2026Review
- Enhancing papaya resistance to ringspot virus through CRISPR/Cas9-mediated gene editing of eFrontiers in plant science · 2026Article
- CRISPR-Cas systems for plant virus management: detection, surveillance, and host resistance.Frontiers in plant science · 2026Review
- Turning Susceptibility into Strength: A New Era of Durable Resistance in Plants Through Genome Editing.Plants (Basel, Switzerland) · 2025Review
- Oligomeric SWEET1g of Solanum tuberosum confers resistance to potato virus Y and Potato virus X.The Plant journal : for cell and molecular biology · 2025Article
- Review
- CRISPR mediated gene editing for economically important traits in horticultural crops: progress and prospects.Transgenic research · 2025Review
- Validation of CRISPR construct activity and gene function in melon via a hairy root transformation system.Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Article
- CRISPR/Cas9-mediated editing of eukaryotic elongation factor 1B gamma (eEF1Bγ) reduces Tobacco etch virus accumulation in Nicotiana benthamiana.Plant cell reports · 2025Article
- CRISPR-mediated genome editing of wheat for enhancing disease resistance.Frontiers in genome editing · 2025Review
- Application of genome editing in plant reproductive biology: recent advances and challenges.Plant reproduction · 2024Review
- EditingInternational journal of molecular sciences · 2024Article
- Rice Yellow Mottle Virus resistance by genome editing of the Oryza sativa L. ssp. japonica nucleoporin gene OsCPR5.1 but not OsCPR5.2.Plant biotechnology journal · 2024Article
- Developmental stages and episode-specific regulatory genes in andromonoecious melon flower development.Annals of botany · 2024Article
- Crop bioengineering via gene editing: reshaping the future of agriculture.Plant cell reports · 2024Review
- A natural substitution of a conserved amino acid in eIF4E confers resistance against multiple potyviruses.Molecular plant pathology · 2024Article
- Improvement of crop production in controlled environment agriculture through breeding.Frontiers in plant science · 2024Review
- CRISPR-Cas systems and applications for crop bioengineering.Frontiers in bioengineering and biotechnology · 2024Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cap-binding protein eIF4E, through its interaction with eIF4G, constitutes the core of the eIF4F complex, which plays a key role in the circularization of mRNAs and their subsequent cap-dependent translation. In addition to its fundamental role in mRNA translation initiation, other functions have been described or suggested for eIF4E, including acting as a proviral factor and participating in sexual development. We used CRISPR/Cas9 genome editing to generate melon eif4e knockout mutant lines. Editing worked efficiently in melon, as we obtained transformed plants with a single-nucleotide deletion in homozygosis in the first eIF4E exon already in a T0 generation. Edited and non-transgenic plants of a segregating F2 generation were inoculated with Moroccan watermelon mosaic virus (MWMV); homozygous mutant plants showed virus resistance, while heterozygous and non-mutant plants were infected, in agreement with our previous results with plants silenced in eIF4E. Interestingly, all homozygous edited plants of the T0 and F2 generations showed a male sterility phenotype, while crossing with wild-type plants restored fertility, displaying a perfect correlation between the segregation of the male sterility phenotype and the segregation of the eif4e mutation. Morphological comparative analysis of melon male flowers along consecutive developmental stages showed postmeiotic abnormal development for both microsporocytes and tapetum, with clear differences in the timing of tapetum degradation in the mutant versus wild-type. An RNA-Seq analysis identified critical genes in pollen development that were down-regulated in flowers of eif4e/eif4e plants, and suggested that eIF4E-specific mRNA translation initiation is a limiting factor for male gametes formation in melon.
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Registered trials
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