Evidence map›Paper›PMID 35778687›Full record

ArticleBMC genomics2022

Transcriptome analysis of the response to low temperature acclimation in Calliptamus italicus eggs.

Qian Liu, Di Luo, Mengjia Wang, Xingmin Song, Xiaofang Ye, Roman Jashenko, Rong Ji

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC genomics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
2.4field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 14 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 2 countries.

Qian LiuInternational Center for the Collaborative Management of Cross-border Pest in Central Asia, Xinjiang Key Laboratory of Special Species Conservation and Regulatory Biology, Xinjiang Normal University, Urumqi, 830054, China.
Di LuoInternational Center for the Collaborative Management of Cross-border Pest in Central Asia, Xinjiang Key Laboratory of Special Species Conservation and Regulatory Biology, Xinjiang Normal University, Urumqi, 830054, China.
Mengjia WangInternational Center for the Collaborative Management of Cross-border Pest in Central Asia, Xinjiang Key Laboratory of Special Species Conservation and Regulatory Biology, Xinjiang Normal University, Urumqi, 830054, China.
Xingmin SongInternational Center for the Collaborative Management of Cross-border Pest in Central Asia, Xinjiang Key Laboratory of Special Species Conservation and Regulatory Biology, Xinjiang Normal University, Urumqi, 830054, China.
Xiaofang YeInternational Center for the Collaborative Management of Cross-border Pest in Central Asia, Xinjiang Key Laboratory of Special Species Conservation and Regulatory Biology, Xinjiang Normal University, Urumqi, 830054, China.
Roman JashenkoAl-Farabi Kazakh National University, Almaty, Kazakhstan, 050038.
Rong JiInternational Center for the Collaborative Management of Cross-border Pest in Central Asia, Xinjiang Key Laboratory of Special Species Conservation and Regulatory Biology, Xinjiang Normal University, Urumqi, 830054, China. jirong@xjnu.edu.cn.
Xinjiang Normal University · CNAl-Farabi Kazakh National University · KZ

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundCalliptamus italicus is a dominant species in the desert and semi-desert grassland. It is widely distributed throughout many regions such as Asia, Europe, North Africa and the Mediterranean, and has enormous destructive potential for agriculture and animal husbandry. The C. italicus overwintering as eggs in the soil through diapause, and the cold tolerance of locust eggs is the key to their ability to survive the winter smoothly to maintain the population.

resultsTranscriptome analysis of C. italicus eggs was carried out in this paper in constant low temperature acclimation, natural low temperature acclimation and room temperature. The differentially expressed genes related to cold tolerance were screened out, the differences in expression patterns under different low temperature acclimation were analyzed, and the genes in the significantly up-regulated pathways may play an important role in cold tolerance. The results show that different domestication modes can induce C. italicus eggs to express a large number of genes to alleviate low temperature damage, but C. italicus eggs are more sensitive to changes in temperature. Compared with the control, there are 8689 DEGs at constant low temperature and 14,994 DEGs at natural low temperature. KEGG analysis showed that DEGs were mainly enriched in pathways related to metabolism and biological systems under constant low temperature, and were mainly enriched in pathways related to biological systems and environmental information processing under natural low temperature. In addition, RNAi technology was used to further verify the regulation of genes in the significantly enriched up-regulated pathways on C. italicus eggs, and it was confirmed that the hatching rate of C. italicus eggs at low temperature was significantly reduced after interference.

conclusionsTranscriptome analysis of C. italicus eggs treated at different temperatures provided a theoretical basis for further understanding the adaptation mechanism of C. italicus eggs to low temperature. In addition, four potential RNAi target genes were verified in the eggs of C. italicus for the first time, providing new ideas for effective control of this species.

Indexed as

Gene Expression ProfilingGrasshoppersAcclimatizationAnimalsCold TemperatureTemperatureCalliptamus italicusCold toleranceLow temperature acclimationRNA interferenceTranscriptome

Identifiers

PMID35778687
PMCPMC9248191
OpenAlexW4283781769

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.