ArticleACS applied materials & interfaces2022
Non-Invasive Three-Dimensional Cell Analysis in Bioinks by Raman Imaging.
Article in ACS applied materials & interfaces, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed, 26 citations in OpenAlex.
- Confocal Raman Microscopy Quantitatively Reveals Subcellular Metabolic Adaptation Mechanisms of Deep-Sea Mussels to Methane Deprivation.Environmental science & technology · 2026Article
- Article
- Noninvasive andThe Analyst · 2025Article
- Raman analysis of lipids in cells: Current applications and future prospects.Journal of pharmaceutical analysis · 2025Review
- Toward robust and reproducible pluripotent stem cell expansion in bioprinted GelMA constructs.International journal of bioprinting · 2025Article
- Biomaterials Mimicking Mechanobiology: A Specific Design for a Specific Biological Application.International journal of molecular sciences · 2024Review
- Translating genomic tools to Raman spectroscopy analysis enables high-dimensional tissue characterization on molecular resolution.Nature communications · 2023Article
- Article
- Current strategies with implementation of three-dimensional cell culture: the challenge of quantification.Interface focus · 2022Review
- Evaluating the Suitability of 3D Bioprinted Samples for Experimental Radiotherapy: A Pilot Study.International journal of molecular sciences · 2022Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
3D bioprinting is an emerging biofabrication strategy using bioinks, comprising cells and biocompatible materials, to produce functional tissue models. Despite progress in building increasingly complex objects, biological analyses in printed constructs remain challenging. Especially, methods that allow non-invasive and non-destructive evaluation of embedded cells are largely missing. Here, we implemented Raman imaging for molecular-sensitive investigations on bioprinted objects. Different aspects such as culture formats (2D, 3D-cast, and 3D-printed), cell types (endothelial cells and fibroblasts), and the selection of the biopolymer (alginate, alginate/nanofibrillated cellulose, alginate/gelatin) were considered and evaluated. Raman imaging allowed for marker-independent identification and localization of subcellular components against the surrounding biomaterial background. Furthermore, single-cell analysis of spectral signatures, performed by multivariate analysis, demonstrated discrimination between endothelial cells and fibroblasts and identified cellular features influenced by the bioprinting process. In summary, Raman imaging was successfully established to analyze cells in 3D culture in situ and evaluate them with regard to the localization of different cell types and their molecular phenotype as a valuable tool for quality control of bioprinted objects.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.