ArticleSTAR protocols2022
A beginner's guide to assembling a draft genome and analyzing structural variants with long-read sequencing technologies.
Article in STAR protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed.
- ONT-only genome assembly of a Korean male individual using a semen sample.Genes & genomics · 2026Article
- Conspecific Scaffold-Level Genome Assembly Outperforms Heterospecific Chromosome-Level Assemblies for Assessing Genetic Indicators in a Threatened Marine Fish.Evolutionary applications · 2026Article
- Resolving repetitive and telomeric regions through a high-contiguity genome assembly of the African Turquoise killifish.Genes & genomics · 2026Article
- Chemically guided single-cell transcriptomics reveals sulfotransferase-mediated scaffold remodeling in securinine biosynthesis.Nature communications · 2026Article
- In Silico Design and Characterization of a Rationally Engineered Cas12j2 Gene Editing System for the Treatment of HPV-Associated Cancers.International journal of molecular sciences · 2026Article
- ChromoMapper: a new tool to quickly compare large genome assemblies.Bioinformatics advances · 2026Article
- Homology-mediated transformation of frog-killing fungusProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Establishing genome sequencing and assembly for non-model and emerging model organisms: a brief guide.Frontiers in zoology · 2025Review
- Units containing telomeric repeats are prevalent in subtelomeric regions of a Mesorhabditis isolate collected from the Republic of Korea.Genes & genomics · 2024Article
- Fully phased genome assemblies and graph-based genetic variants of the olive flounder, Paralichthys olivaceus.Scientific data · 2024Article
- Genome assembly of Hibiscus sabdariffa L. provides insights into metabolisms of medicinal natural products.G3 (Bethesda, Md.) · 2024Article
- Whole-genome sequencing of 13 Arctic plants and draft genomes of Oxyria digyna and Cochlearia groenlandica.Scientific data · 2024Article
- Chromosome-Level Assemblies of the Pieris mannii Butterfly Genome Suggest Z-Origin and Rapid Evolution of the W Chromosome.Genome biology and evolution · 2023Article
- Targeted gene deletion with SpCas9 and multiple guide RNAs in Arabidopsis thaliana: four are better than two.Plant methods · 2023Article
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2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Advances in long-read DNA sequencing technologies have enabled researchers to obtain high-quality genomes and finely resolve structural variants (SVs) in many species, even from small laboratories. The hands-on protocol presented here will guide you through the process of analyzing three different types of publicly available Drosophila melanogaster datasets obtained using current long-read sequencing technologies. We hope that this protocol will help in guiding researchers who are new to the process of long-read sequencing analysis.
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