Evidence map›Paper›PMID 35776323›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Enhanced Transduction of P2X7-Expressing Cells with Recombinant rAAV Vectors.

Anna Marei Mann, Waldemar Schäfer, Sahil Adriouch, Kathleen Börner, Dirk Grimm, Ingke Braren, Friedrich Koch-Nolte

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
1.1field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 5 institutions in 2 countries.

Anna Marei Mann *Institute of Immunology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
Waldemar Schäfer *Institute of Immunology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
Sahil AdriouchUNIROUEN, INSERM, U1234, Pathophysiology, Autoimmunity, Neuromuscular Diseases and Regenerative THERapies (PANTHER), Normandie University, Rouen, France.
Kathleen BörnerDepartment of Infectious Diseases/Virology, Medical Faculty, Heidelberg University, Heidelberg, Germany.
Dirk GrimmDepartment of Infectious Diseases/Virology, Medical Faculty, Heidelberg University, Heidelberg, Germany.
Ingke BrarenInstitute of Experimental Pharmacology and Toxicology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
Friedrich Koch-NolteInstitute of Immunology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany. nolte@uke.de.
Universität Hamburg · DEGerman Center for Infection Research · DEPhysiopathologie, autoimmunité, maladies neuromusculaire et thérapie régénératriceUniversity Hospital Heidelberg · DEUniversity Medical Center Hamburg-Eppendorf · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Adeno-associated viruses (AAV) are useful vectors for transducing cells in vitro and in vivo. Targeting of specific cell subsets with AAV is limited by the broad tropism of AAV serotypes. Nanobodies are single immunoglobulin variable domains from heavy chain antibodies that naturally occur in camelids. Their small size and high solubility allow easy reformatting into fusion proteins. In this chapter we provide protocols for inserting a P2X7-specific nanobody into a surface loop of the VP1 capsid protein of AAV2. Such nanobody-displaying recombinant AAV allow 50- to 500-fold stronger transduction of P2X7-expressing cells than the parental AAV. We provide protocols for monitoring the transduction of P2X7-expressing cells by nanobody-displaying rAAV by flow cytometry and fluorescence microscopy.

Indexed as

DependovirusGenetic VectorsCapsid ProteinsTransduction, GeneticTropismCapsid ProteinsAAVAdeno-associated viral vectorsFlow cytometryFluorescence microscopyP2X7Western blot

Identifiers

PMID35776323
OpenAlexW4285235941

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.