ArticleThe Plant cell2022
The plant ESCRT component FREE1 regulates peroxisome-mediated turnover of lipid droplets in germinating Arabidopsis seedlings.
Article in The Plant cell, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 25 citations in OpenAlex.
- Building peroxisomes: perspectives on plant peroxins.Biochemical Society transactions · 2026Review
- Functional dissection of theaBIOTECH · 2026Article
- Electron microscopy-based three-dimensional subcellular imaging of plant male gametophyte.Journal of integrative plant biology · 2026Review
- PEX11 mediates intralumenal vesicle formation in peroxisomes.Nature communications · 2026Article
- Review
- Lipid-sugar conversion regulates early white clover (Trifolium repens) seedling establishment under cadmium stress.Planta · 2025Article
- The green ESCRTs: Newly defined roles for ESCRT proteins in plants.The Journal of biological chemistry · 2025Review
- The Atypical Pectin Methylesterase Family Member PME31 Promotes Seedling Lipid Droplet Utilization.Plant direct · 2025Article
- The Use of Arabidopsis thaliana to Characterize the Production and Action Stages of the Plant MicroRNA Pathway.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Article
- Lipid droplets in plants: turnover and stress responses.Frontiers in plant science · 2025Review
- Developmental pleiotropy of SDP1 from seedling to mature stages in B. napus.Plant molecular biology · 2024Article
- Autophagy in plants.Autophagy reports · 2024Review
- Whole-Cell Electron Tomography Analysis of Vacuole Biogenesis.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Article
- Molecular Machinery of Lipid Droplet Degradation and Turnover in Plants.International journal of molecular sciences · 2023Review
- The ubiquitin-protein ligase MIEL1 localizes to peroxisomes to promote seedling oleosin degradation and lipid droplet mobilization.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Lipid droplets are versatile organelles involved in plant development and plant response to environmental changes.Frontiers in plant science · 2023Review
- Identification and characterization of the LDAP family revealedFrontiers in plant science · 2023Article
- FREE1 takes its position in peroxisomal engulfment of lipid droplets.The Plant cell · 2022Article
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Authors and funding
14 authors at 7 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lipid droplets (LDs) stored during seed development are mobilized and provide essential energy and lipids to support seedling growth upon germination. Triacylglycerols (TAGs) are the main neutral lipids stored in LDs. The lipase SUGAR DEPENDENT 1 (SDP1), which hydrolyzes TAGs in Arabidopsis thaliana, is localized on peroxisomes and traffics to the LD surface through peroxisomal extension, but the underlying mechanism remains elusive. Here, we report a previously unknown function of a plant-unique endosomal sorting complex required for transport (ESCRT) component FYVE DOMAIN PROTEIN REQUIRED FOR ENDOSOMAL SORTING 1 (FREE1) in regulating peroxisome/SDP1-mediated LD turnover in Arabidopsis. We showed that LD degradation was impaired in germinating free1 mutant; moreover, the tubulation of SDP1- or PEROXIN 11e (PEX11e)-marked peroxisomes and the migration of SDP1-positive peroxisomes to the LD surface were altered in the free1 mutant. Electron tomography analysis showed that peroxisomes failed to form tubules to engulf LDs in free1, unlike in the wild-type. FREE1 interacted directly with both PEX11e and SDP1, suggesting that these interactions may regulate peroxisomal extension and trafficking of the lipase SDP1 to LDs. Taken together, our results demonstrate a pivotal role for FREE1 in LD degradation in germinating seedlings via regulating peroxisomal tubulation and SDP1 targeting.
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