ArticleJournal of periodontal & implant science2022
Bio-hybrid dental implants prepared using stem cells with β-TCP-coated titanium and zirconia.
Article in Journal of periodontal & implant science, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
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Who cites it
15 citing papers in PubMed, 1 synthesis or guideline pooled it, 27 citations in OpenAlex.
- Root Cementum Molecular Structure and Its Role in Maintaining Oral Health-Systematic Review.International journal of molecular sciences · 2025Pooled it
- An Implanted Tooth That Can Feel.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Anodization and Its Role in Peri-Implant Tissue Adhesion: A Novel 3D Bioprinting Approach.Journal of functional biomaterials · 2026Article
- Enhancing Osseointegration of Zirconia Implants Using Calcium Phosphate Coatings: A Systematic Review.Materials (Basel, Switzerland) · 2025Review
- The Future of Dental Implants: A Narrative Review of Trends, Technologies, and Patient Considerations.Cureus · 2025Review
- Regenerative Strategies in Dentistry: Harnessing Stem Cells, Biomaterials and Bioactive Materials for Tissue Repair.Biomolecules · 2025Review
- Enhancement of Chondrogenic Differentiation in Bone Marrow-Derived Stem Cell Spheroids byJournal of personalized medicine · 2024Article
- Promoting and accelerating muscle regeneration through cell therapy in a mouse model.Journal of Taibah University Medical Sciences · 2024Article
- Evaluation of Random and Aligned Polycaprolactone Nanofibrous Electrospun Scaffold for Human Periodontal Ligament Engineering in Biohybrid Titanium Implants.International journal of dentistry · 2024Article
- Spatiotemporal cellular dynamics and molecular regulation of tooth root ontogeny.International journal of oral science · 2023Review
- Sinus augmentation with poly(ε)caprolactone-β tricalcium phosphate scaffolds, mesenchymal stem cells and platelet rich plasma for one-stage dental implantation in minipigs.Journal of periodontal & implant science · 2023Article
- Vitamin E Enhances Cell Viability and the Osteogenic Differentiation of Cell Spheroids Made of Gingiva-Derived Stem Cells.Medicina (Kaunas, Lithuania) · 2023Article
- Effects of Enamel Matrix Derivative on Cell Spheroids Made of Stem Cells Obtained from the Gingiva on Osteogenic Differentiation.Medicina (Kaunas, Lithuania) · 2023Article
- Influence of Fiber Laser (1064 nm) on Shear Bond Strength of Titanium Abutment and Resin Cement.Journal of lasers in medical sciences · 2022Article
- Innovations in Dental Implants Integration: Optimizing dental implants performance utilizing stem cells and coatings.Biotechnology progressReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeThis study investigated periodontal ligament (PDL) restoration in osseointegrated implants using stem cells.
methodsCommercial pure titanium and zirconium oxide (zirconia) were coated with beta-tricalcium phosphate (β-TCP) using a long-pulse Nd:YAG laser (1,064 nm). Isolated bone marrow mesenchymal cells (BMMSCs) from rabbit tibia and femur, isolated PDL stem cells (PDLSCs) from the lower right incisor, and co-cultured BMMSCs and PDLSCs were tested for periostin markers using an immunofluorescent assay. Implants with 3D-engineered tissue were implanted into the lower right central incisors after extraction from rabbits. Forty implants (Ti or zirconia) were subdivided according to the duration of implantation (healing period: 45 or 90 days). Each subgroup (20 implants) was subdivided into 4 groups (without cells, PDLSC sheets, BMMSC sheets, and co-culture cell sheets). All groups underwent histological testing involving haematoxylin and eosin staining and immunohistochemistry, stereoscopic analysis to measure the PDL width, and field emission scanning electron microscopy (FESEM). The natural lower central incisors were used as controls.
resultsThe BMMSCs co-cultured with PDLSCs generated a well-formed PDL tissue that exhibited positive periostin expression. Histological analysis showed that the implantation of coated (Ti and zirconia) dental implants without a cell sheet resulted in a well-osseointegrated implant at both healing intervals, which was confirmed with FESEM analysis and negative periostin expression. The mesenchymal tissue structured from PDLSCs only or co-cultured (BMMSCs and PDLSCs) could form a natural periodontal tissue with no significant difference between Ti and zirconia implants, consequently forming a biohybrid dental implant. Green fluorescence for periostin was clearly detected around the biohybrid implants after 45 and 90 days. FESEM showed the invasion of PDL-like fibres perpendicular to the cementum of the bio-hybrid implants.
conclusionsβ-TCP-coated (Ti and zirconia) implants generated periodontal tissue and formed biohybrid implants when mesenchymal-tissue-layered cell sheets were isolated from PDLSCs alone or co-cultured BMMSCs and PDLSCs.
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