ArticleApplications in plant sciences
Ancient DNA extraction methods for herbarium specimens: When is it worth the effort?
Article in Applications in plant sciences. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 35 citations in OpenAlex.
- The Herbarium of Paolo Boccone from the Years 1689-1695 Kept in Wrocław (Poland): Plant Nomenclature, Taxonomy and Biogeography.Plants (Basel, Switzerland) · 2026Article
- The utility of herbarium collections for genetic monitoring.Bioscience · 2026Review
- Ancient wheat DNA: How and what for?Journal, genetic engineering & biotechnology · 2026Article
- Exploring the Quality of Historic DNA From Pinned Beetles.Ecology and evolution · 2026Article
- Herbariomic approach solved identity crisis of the putatively extinct Armeria arcuata Welw. ex Boiss. & Reut. (Plumbaginaceae).Annals of botany · 2026Article
- Whole genome sequencing of historical specimens from the world's largest fungal collection yields high-quality assemblies.The New phytologist · 2026Article
- From Permits to Samples: Addressing Key Challenges for High-Quality Reference Genome Generation in Europe.Molecular ecology resources · 2026Review
- Optimizing ancient DNA recovery from archaeological plant seeds.Scientific reports · 2025Article
- A Computer Vision Method for Finding Mislabelled Specimens Within Natural History Collections.Ecology and evolution · 2025Article
- Are Ophiolitic Substrates Drivers for Reticulate Evolution inEcology and evolution · 2025Article
- Versatile DNA extraction from diverse plant taxa using ionic liquids and magnetic ionic liquids: a methodological breakthrough for enhanced sample utility.Plant methods · 2024Article
- From Dormant Collections to Repositories for the Study of Habitat Changes: The Importance of Herbaria in Modern Life Sciences.Life (Basel, Switzerland) · 2023Review
- Retrieval of long DNA reads from herbarium specimens.AoB PLANTS · 2023Review
- Challenges and Opportunities behind the Use ofPlants (Basel, Switzerland) · 2023Review
- Check Your Shopping Cart: DNA Barcoding and Mini-Barcoding for Food Authentication.Foods (Basel, Switzerland) · 2023Article
- Ancient DNA extraction methods for herbarium specimens: When is it worth the effort?Applications in plant sciencesArticle
- A comparison of freezer-stored DNA and herbarium tissue samples for chloroplast assembly and genome skimming.Applications in plant sciencesArticle
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Premise: Herbaria harbor a tremendous number of plant specimens that are rarely used for molecular systematic studies, largely due to the difficulty in extracting sufficient amounts of high-quality DNA from the preserved plant material. Methods: We compared the standard Qiagen DNeasy Plant Mini Kit and a specific protocol for extracting ancient DNA (aDNA) (the Results: The PTB-DTT method produced a higher DNA yield of better quality than the Qiagen kit; however, extracts from the Qiagen kit over a certain DNA yield and quality threshold produced comparable sequencing results. The sequencing resulted in high proportions of endogenous reads. We were able to successfully sequence 200-year-old samples. Discussion: This method comparison revealed that, for younger specimens, DNA extraction using a standard kit might be sufficient. For old and precious herbarium specimens, aDNA extraction methods are better suited to meet the requirements for next-generation sequencing.
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