ArticleActa informatica medica : AIM : journal of the Society for Medical Informatics of Bosnia & Herzegovina : casopis Drustva za medicinsku informatiku BiH2022
The Effectiveness of Sperm Preparation Using Density Mini-Gradient and Single-Layer Centrifugation for Oligospermia Samples.
Article in Acta informatica medica : AIM : journal of the Society for Medical Informatics of Bosnia & Herzegovina : casopis Drustva za medicinsku informatiku BiH, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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2 citing papers in PubMed.
- Exploring the impact of the common (4977 bp) and ATPase mitochondrial DNA deletion on the copy number and telomere length in a sample of infertile Iraqi men.Molecular biology reports · 2026Article
- Timing Matters? Impact of Early Sperm Processing on Motile Sperm Recovery, Fertilization, Blastocyst Rate, and Pregnancy Outcomes in IUI and IVF.Journal of clinical medicine · 2025Article
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7 authors.
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Abstract
Background: Sperm preparation is an important step during assisted reproduction, and different assisted reproductive techniques have different sperm quality requirements. For intrauterine insemination (IUI), the total motile sperm count is a predictor of a patient's fertility. Objective: The aim of this study was to compare the sperm recovery rate and DNA fragmentation index (DFI) outcomes following density mini-gradient and single-layer centrifugation in preparation for intrauterine insemination (IUI). Methods: A total of 30 semen samples with concentrations under 15 million cells/ml were obtained, and each sample was divided into 3 aliquots, with each aliquot subjected to 1 of 3 separation methods: mini-gradient, single-layer using a 90% density layer (single 90-layer), and single-layer using a 45% density layer (single 45-layer). Total sperm motility and sperm recovery rates were compared before and after preparation using each method. Results: The sperm concentration obtained using single 45-layer was higher than the other groups (p<0.05), but sperm motility was higher using the mini-gradient and single 90-layer methods higher than the single 45-layer method (p<0.05). The recovered sperm motility rates for the mini-gradient, single 90-layer, and single 45-layer methods were 57.6% ± 20.6%, 62.8% ± 18.5%, and 78.7% ± 12.4%, respectively, indicating a better outcome for the single 45-layer method than for the other methods. Conclusion: All of these methods can be applied to sperm preparation for IUI, and the optimal method can be selected based on initial sperm quality to collect sperm with good motility and DNA integrity to achieve a satisfactory pregnancy rate.
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