ArticleBiomedical optics express2022
Photon efficient orientation estimation using polarization modulation in single-molecule localization microscopy.
Article in Biomedical optics express, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Optimal excitation polarization schemes to boost the precision of single-molecule orientation imaging.Biomedical optics express · 2026Article
- Single Fluorogens and Orientation-Localization Microscopy for Quantifying Chemical and Biomolecular Dynamics at the Nanoscale.Accounts of chemical research · 2026Article
- Emission dipole orientation reveals dynamic single-molecule interactions with 2D crystals at solvent interfaces.Nature communications · 2026Article
- Dual-view microscopy of single-molecule dipole orientations.Biophysical reports · 2025Article
- Single molecule orientation and localization microscopy.Nature photonics · 2025Article
- Painting rich six-dimensional pictures using polarized fluorescence microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Resolving the Orientations of and Angular Separation Between a Pair of Dipole Emitters.Physical review letters · 2025Article
- Single-molecule orientation-localization microscopy: Applications and approaches.Quarterly reviews of biophysics · 2024Review
- Fundamental Limits in Measuring the Anisotropic Rotational Diffusion of Single Molecules.The journal of physical chemistry. A · 2024Article
- Six-Dimensional Single-Molecule Imaging with Isotropic Resolution using a Multi-View Reflector Microscope.Nature photonics · 2023Article
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Authors and funding
4 authors.
Funding
Abstract
Combining orientation estimation with localization microscopy opens up the possibility to analyze the underlying orientation of biomolecules on the nanometer scale. Inspired by the recent improvement of the localization precision by shifting excitation patterns (MINFLUX, SIMFLUX), we have adapted the idea towards the modulation of excitation polarization to enhance the orientation precision. For this modality two modes are analyzed: i) normally incident excitation with three polarization steps to retrieve the in-plane angle of emitters and ii) obliquely incident excitation with p-polarization with five different azimuthal angles of incidence to retrieve the full orientation. Firstly, we present a theoretical study of the lower precision limit with a Cramér-Rao bound for these modes. For the oblique incidence mode we find a favorable isotropic orientation precision for all molecular orientations if the polar angle of incidence is equal to
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