ArticleJournal of biomedical optics2022
Functional imaging with dynamic quantitative oblique back-illumination microscopy.
Article in Journal of biomedical optics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 27 citations in OpenAlex.
- Foundation model cascades enable zero-shot microscopy image analysis for cell therapy manufacturing.Cytotherapy · 2026Article
- Towards a mobile quantitative phase imaging microscope with smartphone phase-detection sensors.Biomedical optics express · 2026Article
- Single capture quantitative oblique back-illumination microscopy.Npj imaging · 2026Article
- Article
- Nondestructive, High-Resolution T Cell Characterization and Subtyping via Deep-UV Microscopy.BME frontiers · 2026Article
- Automated vertical wheel bioreactor integrated with process analytics for T-cell manufacturing.Cytotherapy · 2025Article
- Label-free in-line characterization of immune cell culture using quantitative phase imaging.NPJ Regenerative medicine · 2025Article
- Experimental assessment of the optical transfer function for quantitative oblique back illumination microscopy (qOBM).Optics express · 2025Article
- Acetic acid enabled nuclear contrast enhancement in epi-mode quantitative phase imaging.Journal of biomedical optics · 2025Article
- Non-invasive label-free imaging analysis pipeline for in situ characterization of 3D brain organoids.Scientific reports · 2024Article
- Non-Invasive Label-free Analysis Pipeline for In Situ Characterization of Differentiation in 3D Brain Organoid Models.Research square · 2024Article
- Label-free functional analysis of root-associated microbes with dynamic quantitative oblique back-illumination microscopy.Scientific reports · 2024Article
- Real-time semantic segmentation and anomaly detection of functional images for cell therapy manufacturing.Cytotherapy · 2023Article
- Label-Free Functional Analysis of Root-Associated Microbes with Dynamic Quantitative Oblique Back-illumination Microscopy.Research square · 2023Article
- Analysis of structural effects of sickle cell disease on brain vasculature of mice using three-dimensional quantitative phase imaging.Journal of biomedical optics · 2023Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
significanceQuantitative oblique back-illumination microscopy (qOBM) is a recently developed label-free imaging technique that enables 3D quantitative phase imaging of thick scattering samples with epi-illumination. Here, we propose dynamic qOBM to achieve functional imaging based on subcellular dynamics, potentially indicative of metabolic activity. We show the potential utility of this novel technique by imaging adherent mesenchymal stromal cells (MSCs) grown in bioreactors, which can help address important unmet needs in cell manufacturing for therapeutics.
aimWe aim to develop dynamic qOBM and demonstrate its potential for functional imaging based on cellular and subcellular dynamics. APPROACH: To obtain functional images with dynamic qOBM, a sample is imaged over a period of time and its temporal signals are analyzed. The dynamic signals display an exponential frequency response that can be analyzed with phasor analysis. Functional images of the dynamic signatures are obtained by mapping the frequency dynamic response to phasor space and color-coding clustered signals.
resultsFunctional imaging with dynamic qOBM provides unique information related to subcellular activity. The functional qOBM images of MSCs not only improve conspicuity of cells in complex environments (e.g., porous micro-carriers) but also reveal two distinct cell populations with different dynamic behavior.
conclusionsIn this work we present a label-free, fast, and scalable functional imaging approach to study and intuitively display cellular and subcellular dynamics. We further show the potential utility of this novel technique to help monitor adherent MSCs grown in bioreactors, which can help achieve quality-by-design of cell products, a significant unmet need in the field of cell therapeutics. This approach also has great potential for dynamic studies of other thick samples, such as organoids.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.