Evidence map›Paper›PMID 35773575›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Membrane Protein Production in Escherichia coli: Protocols and Rules.

Jordi Royes, Pauline Talbot, Christel Le Bon, Karine Moncoq, Marc Uzan, Francesca Zito, Bruno Miroux

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
3.3field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Jordi RoyesLaboratoire de Colloïdes et Matériaux Divisés École Supérieure de Physique et de Chimie Industrielles de la Ville de Paris 10, Paris, France.
Pauline TalbotUniversité Paris Cité, CNRS, Laboratoire de Biologie Physico-Chimique des Protéines Membranaires, Institut de Biologie Physico-Chimique, Paris, France.
Christel Le BonUniversité Paris Cité, CNRS, Laboratoire de Biologie Physico-Chimique des Protéines Membranaires, Institut de Biologie Physico-Chimique, Paris, France.
Karine MoncoqUniversité Paris Cité, CNRS, Laboratoire de Biologie Physico-Chimique des Protéines Membranaires, Institut de Biologie Physico-Chimique, Paris, France.
Marc UzanUniversité Paris Cité, CNRS, Laboratoire de Biologie Physico-Chimique des Protéines Membranaires, Institut de Biologie Physico-Chimique, Paris, France.
Francesca ZitoUniversité Paris Cité, CNRS, Laboratoire de Biologie Physico-Chimique des Protéines Membranaires, Institut de Biologie Physico-Chimique, Paris, France.
Bruno MirouxUniversité Paris Cité, CNRS, Laboratoire de Biologie Physico-Chimique des Protéines Membranaires, Institut de Biologie Physico-Chimique, Paris, France. Bruno.Miroux@ibpc.fr.
Centre National de la Recherche Scientifique · FRUniversité Paris Cité · FRUniversité Paris Nanterre · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Despite recent progresses in the use of eukaryotic expression system, production of membrane proteins for structural studies still relies on microbial expression systems. In this review, we provide protocols to achieve high level expression of membrane proteins in Escherichia coli, especially using the T7 RNA polymerase based expression system. From the design of the construct, the choice of the appropriate vector-host combination, the assessment of the bacterial fitness, to the selection of bacterial mutant adapted to the production of the target membrane protein, the chapter covers all necessary methods for a rapid optimization of a specific target membrane protein. In addition, we provide a protocol for membrane protein solubilization based on our recent analysis of the Protein Data Bank.

Indexed as

Escherichia coliMembrane ProteinsDNA-Directed RNA PolymerasesRecombinant ProteinsReview Literature as TopicDNA-Directed RNA PolymerasesMembrane ProteinsRecombinant ProteinsE. coliMembrane proliferationMembrane proteinsProduction of recombinant proteinsT7 RNA polymerase

Identifiers

PMID35773575
OpenAlexW4285185160

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.