ArticleLight, science & applications2022
An alternative to MINFLUX that enables nanometer resolution in a confocal microscope.
Article in Light, science & applications, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 21 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 54 citations in OpenAlex.
- Nanorulers for Super-Resolution Microscopy.Chembiochem : a European journal of chemical biology · 2026Review
- Open-source sub-nanometer stabilization system for super-resolution fluorescence microscopy.Light, science & applications · 2025Article
- Cellular optical imaging techniques: a dynamic advancing frontier.Science China. Life sciences · 2025Review
- Localization of single molecules with structured illumination and structured detection.Light, science & applications · 2025Article
- Wide-field fluorescence lifetime imaging of single molecules with a gated single-photon camera.Light, science & applications · 2025Article
- Resolving the structural basis of therapeutic antibody function in cancer immunotherapy with RESI.Nature communications · 2025Article
- Array detection enables large localization range for simple and robust MINFLUX.Light, science & applications · 2025Article
- Open-source 3D active sample stabilization for fluorescence microscopy.Biophysical reports · 2025Article
- MINFLUX nanoscopy enhanced with high-order vortex beams.Light, science & applications · 2025Article
- Review
- MINFLUX achieves molecular resolution with minimal photons.Nature photonics · 2025Article
- Revealing mitochondrial architecture and functions with single molecule localization microscopy.Biology of the cell · 2025Review
- Point-Spread Function Deformations Unlock 3D Localization Microscopy on Spherical Nanoparticles.ACS nano · 2024Article
- Quantifying the minimum localization uncertainty of image scanning localization microscopy.Biophysical reports · 2024Article
- Article
- Differential Confocal Optical Probes with Optimized Detection Efficiency and Pearson Correlation Coefficient Strategy Based on the Peak-Clustering Algorithm.Micromachines · 2023Article
- Ångström-resolution fluorescence microscopy.Nature · 2023Article
- Combining pMINFLUX, graphene energy transfer and DNA-PAINT for nanometer precise 3D super-resolution microscopy.Light, science & applications · 2023Article
- Raster-scanning Donut simplifies MINFLUX and provides alternative implement on other scanning-based microscopes.Light, science & applications · 2022Article
- Precise Two-Dimensional Tilt Measurement Sensor with Double-Cylindrical Mirror Structure and Modified Mean-Shift Algorithm for a Confocal Microscopy System.Sensors (Basel, Switzerland) · 2022Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Localization of single fluorescent emitters is key for physicochemical and biophysical measurements at the nanoscale and beyond ensemble averaging. Examples include single-molecule tracking and super-resolution imaging by single-molecule localization microscopy. Among the numerous localization methods available, MINFLUX outstands for achieving a ~10-fold improvement in resolution over wide-field camera-based approaches, reaching the molecular scale at moderate photon counts. Widespread application of MINFLUX and related methods has been hindered by the technical complexity of the setups. Here, we present RASTMIN, a single-molecule localization method based on raster scanning a light pattern comprising a minimum of intensity. RASTMIN delivers ~1-2 nm localization precision with usual fluorophores and is easily implementable on a standard confocal microscope with few modifications. We demonstrate the performance of RASTMIN in localization of single molecules and super-resolution imaging of DNA origami structures.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.