Evidence map›Paper›PMID 35772021›Full record

ArticleDiabetes2022

AIFM2 Is Required for High-Intensity Aerobic Exercise in Promoting Glucose Utilization.

Hai P Nguyen, Sneha Damal Villivalam, Byung Chul Jung, Dongjoo You, Frances Lin, Danielle Yi, Anna Pi, Katherine Ma, Sunhee Jung, Sang-Hee Park and 3 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Diabetes, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.2field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 10 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 2 institutions in 1 country.

Hai P NguyenDepartment of Bioengineering and Therapeutic Sciences, University of California, San Francisco, San Francisco, CA.
Sneha Damal VillivalamNutritional Sciences and Toxicology Department, University of California, Berkeley, Berkeley, CA.
Byung Chul JungNutritional Sciences and Toxicology Department, University of California, Berkeley, Berkeley, CA.
Dongjoo YouNutritional Sciences and Toxicology Department, University of California, Berkeley, Berkeley, CA.
Frances LinNutritional Sciences and Toxicology Department, University of California, Berkeley, Berkeley, CA.
Danielle YiNutritional Sciences and Toxicology Department, University of California, Berkeley, Berkeley, CA.
Anna PiNutritional Sciences and Toxicology Department, University of California, Berkeley, Berkeley, CA.
Katherine MaNutritional Sciences and Toxicology Department, University of California, Berkeley, Berkeley, CA.
Sunhee JungDepartment of Biological Chemistry, University of California, Irvine, Irvine, CA.
Sang-Hee ParkDepartment of Biological Chemistry, University of California, Irvine, Irvine, CA.
Cholsoon JangDepartment of Biological Chemistry, University of California, Irvine, Irvine, CA.
Hei Sook SulNutritional Sciences and Toxicology Department, University of California, Berkeley, Berkeley, CA.ORCID 0000-0002-3372-5097
Sona KangNutritional Sciences and Toxicology Department, University of California, Berkeley, Berkeley, CA.ORCID 0000-0002-9831-677X
University of California, Berkeley · USUniversity of California, San Francisco · US

Funding

Stanford Islet Research CoreP30DK116074 · NIDDK · STANFORD UNIVERSITY · PI Seung K Kim · 2017 to 2026
$19.5M
Epigenetic regulation of adipose development and remodelingR01DK116008 · NIDDK · UNIVERSITY OF CALIFORNIA BERKELEY · PI Sona Kang · 2018 to 2026
$3.6M
Brown adipose NADH oxidase for thermogenesisR01DK123843 · NIDDK · UNIVERSITY OF CALIFORNIA BERKELEY · PI SUL, HEI SOOK · 2020 to 2023
$2.4M
Training Program in Metabolic BiologyT32DK128783 · NIDDK · UNIVERSITY OF CALIFORNIA BERKELEY · PI Andreas Stahl · 2022 to 2026
$776k
NIDDK NIH HHS P30 DK116074NIDDK NIH HHS R01 DK116008NIDDK NIH HHS R01 DK123843NIDDK NIH HHS T32 DK128783
6 · The paper itself

Abstract

Skeletal muscle is a major regulator of glycemic control at rest, and glucose utilization increases drastically during exercise. Sustaining a high glucose utilization via glycolysis requires efficient replenishment of NAD+ in the cytosol. Apoptosis-inducing mitochondrion-associated factor 2 (AIFM2) was previously shown to be a NADH oxidoreductase domain-containing flavoprotein that promotes glycolysis for diet and cold-induced thermogenesis. Here, we find that AIFM2 is selectively and highly induced in glycolytic extensor digitorum longus (EDL) muscle during exercise. Overexpression (OE) of AIFM2 in myotubes is sufficient to elevate the NAD+-to-NADH ratio, increasing the glycolytic rate. Thus, OE of AIFM2 in skeletal muscle greatly increases exercise capacity, with increased glucose utilization. Conversely, muscle-specific Aifm2 depletion via in vivo transfection of hairpins against Aifm2 or tamoxifen-inducible haploinsufficiency of Aifm2 in muscles decreases exercise capacity and glucose utilization in mice. Moreover, muscle-specific introduction of NDE1, Saccharomyces cerevisiae external NADH dehydrogenase (NDE), ameliorates impairment in glucose utilization and exercise intolerance of the muscle-specific Aifm2 haploinsufficient mice. Together, we show a novel role for AIFM2 as a critical metabolic regulator for efficient utilization of glucose in glycolytic EDL muscles.

Indexed as

GlucoseNADAnimalsGlycolysisMiceMicrotubule-Associated ProteinsMuscle, SkeletalNADH DehydrogenaseTamoxifenGlucoseMicrotubule-Associated ProteinsNADNADH DehydrogenaseNde1 protein, mouseTamoxifen

Identifiers

PMID35772021
PMCPMC9501658
OpenAlexW4283718517

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.