Detection, Isolation, and Functional Studies of Mouse Pulmonary Group 2 Innate Lymphoid Cells.

Allyssa N Strohm, Taylor A Doherty

Open access · greenAbstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

0.6field-weighted citation impact, top 36% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

Immunopathologic Role of Fungi in Chronic Rhinosinusitis.International journal of molecular sciences · 2023
Review

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Allyssa N StrohmDepartment of Medicine, University of California, San Diego, La Jolla, CA, USA.

Taylor A DohertyDepartment of Medicine, University of California, San Diego, La Jolla, CA, USA. tdoherty@ucsd.edu.

University of California, San Diego · US

Funding

Mentoring patient-oriented researchers in inflammatory airway diseaseK24AI171795 · NIAID · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI TAYLOR A DOHERTY · 2022 to 2026

$968k

Targeting pathogenic innate lymphoid cells in airway diseaseI01BX005073 · VA · VA SAN DIEGO HEALTHCARE SYSTEM · PI TAYLOR A DOHERTY · 2021 to 2026

–

BLRD VA I01 BX005073NIAID NIH HHS K24 AI171795

6 · The paper itself

Abstract

ILC2s are key players in the emergence of type 2 inflammation in many pulmonary diseases. While several phenotypic markers can be used to identify ILC2s, our method utilizes the surface markers CD127 and ST2 to classify a group of type 2 cytokine-producing ILC2s upon activation by the fungal allergen Alternaria alternata . Here, we provide our protocol for the detection and isolation of a highly pure population of pulmonary mouse ILCs via flow cytometry and cell sorting. We also describe the methods for in vitro stimulation to assess the functionality of ILC2s.

Indexed as

Immunity, InnateLymphocytesAnimalsCytokinesInflammationLungMiceCytokinesFACSFlow cytometryILC2ILC2 stainingInnate lymphoid cellsType 2 inflammation

Identifiers

PMID35771471

DOI10.1007/978-1-0716-2364-0_12

PMCPMC9397487

OpenAlexW4285191652

What OpenQuestion holds

Textmetadata

LicenceTDM

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.