Evidence map›Paper›PMID 35771418›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

A Three-Dimensional Culture-Based Assay to Detect Early Stages of Vasculogenic Mimicry in Ovarian Cancer Cells.

Yarely M Salinas-Vera, Dolores Gallardo-Rincón, Erika Ruíz-García, Laurence A Marchat, Jesús Valdés, Carlos Vázquez-Calzada, César López-Camarillo

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
3.6field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 7 citations in OpenAlex.

  1. Biomimetic Scaffold-Based 3D Models for Decoding Cancer Biology and Advancing Therapy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 1 country.

Yarely M Salinas-VeraDepartment of Biochemistry, CINVESTAV-IPN, Ciudad de México, Mexico.
Dolores Gallardo-RincónLaboratory of Translational Medicine and Department of Gastrointestinal Tumors, National Cancer Institute, Ciudad de México, Mexico.
Erika Ruíz-GarcíaLaboratory of Translational Medicine and Department of Gastrointestinal Tumors, National Cancer Institute, Ciudad de México, Mexico.
Laurence A MarchatProgram in Molecular Biomedicine and Biotechnology Network, National Polytechnic Institute, Ciudad de México, Mexico.
Jesús ValdésDepartment of Biochemistry, CINVESTAV-IPN, Ciudad de México, Mexico.
Carlos Vázquez-CalzadaDepartment of Infectomics and Molecular Pathogenesis, CINVESTAV-IPN, Ciudad de México, Mexico.
César López-CamarilloAutonomous University of Mexico City, Ciudad de México, Mexico. cesar.lopez@uacm.edu.mx.
Center for Research and Advanced Studies of the National Polytechnic Institute · MXInstituto Nacional de Cancerología · MXTecnológico Nacional de México · MXUniversidad Autónoma de la Ciudad de México · MX

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Vasculogenic mimicry is a cellular mechanism in which tumor cells grow and align forming complex three-dimensional (3D) channel-like structures in a hypoxic microenvironment. This phenomenon represents a novel oxygen, nutrient, and blood supply, in a similar way as occurs in classic angiogenesis. Vasculogenic mimicry has been described in numerous clinical tumors including breast, prostate, lung, and ovarian cancers where it is associated with poor prognosis; thus, it is considered as a hallmark of highly aggressive and metastatic tumors. Here, we describe a simple method to model the in vitro formation of three-dimensional cellular networks over Matrigel in SKOV3 ovarian cancer cells representing the early stages of vasculogenic mimicry.

Indexed as

Ovarian NeoplasmsCarcinoma, Ovarian EpithelialCell DifferentiationCell Line, TumorFemaleHumansMaleNeovascularization, PathologicTumor MicroenvironmentEngelbrethHolmmicroRNAsmiR-765Ovarian cancerSwarm extracellular matrix extractThree-dimensional cultureVasculogenic mimicry

Identifiers

PMID35771418
OpenAlexW4285223641

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.