ArticleDisease models & mechanisms2022
Exposure to e-cigarette vapor extract induces vocal fold epithelial injury and triggers intense mucosal remodeling.
Article in Disease models & mechanisms, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 24 citations in OpenAlex.
- Association between smoking and vaping behaviors and self-perceived voice handicap in young medical trainees.European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery · 2026Article
- E-cigarette use and Barrett's esophagus: evaluating a new risk in the era of vaping.Esophagus : official journal of the Japan Esophageal Society · 2026Article
- A Human Vocal Fold Organ-On-Chip for Studying Platform-Dependent Mucosal Responses to Particulate Matter.bioRxiv : the preprint server for biology · 2026Article
- Self-reported voice symptoms in e-cigarette users: a cross-sectional study.European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery · 2026Article
- Laryngeal Basal Cell Response to Cigarette Smoke and E-Cigarette Aerosol Exposures.The Laryngoscope · 2026Article
- Cross-kingdom RNA interference in the dysbiosis-mediated network of vocal fold fibrosis: a pathogenic hypothesis and molecular intervention targets.Frontiers in immunology · 2026Review
- Unvaporized e-liquid toxicity elevates CD44-dependent hyaluronan catabolic gene expression and triggers inflammation in human vocal fold fibroblasts.Toxicology reports · 2025Article
- A proposal for epithelial dominance in extremely high fundamental frequency vocalizations.The Journal of the Acoustical Society of America · 2025Article
- The Pediatric Vocal Mechanism: Structure and Function.Journal of voice : official journal of the Voice Foundation · 2025Review
- Chronic airway inflammatory diseases and e-cigarette use: a review of health risks and mechanisms.European journal of medical research · 2025Review
- Knowledge, attitude, and practice toward vocal fatigue among occupational voice users: a cross-sectional study.BMC public health · 2025Article
- Contribution of Streptococcus pseudopneumoniae and Streptococcus salivarius to vocal fold mucosal integrity and function.Disease models & mechanisms · 2024Article
- Effects of Short-term Electronic(e)-Cigarette Aerosol Exposure in the Mouse Larynx.The Laryngoscope · 2024Article
- Mechanistic Insights into Threonine Tyrosine Kinase Mediated Cell Cycle Regulation in Triple-negative Breast Cancer.Cancer genomics & proteomicsArticle
- Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Vaping has been reported to cause acute epiglottitis, a life-threatening airway obstruction induced by direct epithelial injury and subsequent inflammatory reaction. Here, we show that we were able to recapitulate this phenomenon in vitro. Exposure of human engineered vocal fold (VF) mucosae to 0.5% and 5% electronic cigarette (e-cigarette) vapor extract (ECVE) for 1 week induced cellular damage of luminal cells, disrupting homeostasis and innate immune responses. Epithelial erosion was likely caused by accumulation of solvents and lipid particles in the cytosol and intercellular spaces, which altered lipid metabolism and plasma membrane properties. Next, we investigated how the mucosal cells responded to the epithelial damage. We withdrew the ECVE from the experimental system and allowed VF mucosae to regenerate for 1, 3 and 7 days, which triggered intense epithelial remodeling. The epithelial changes included expansion of P63 (TP63)-positive basal cells and cytokeratin 14 (KRT14) and laminin subunit α-5 (LAMA5) deposition, which might lead to local basal cell hyperplasia, hyperkeratinization and basement membrane thickening. In summary, vaping presents a threat to VF mucosal health and airway protection, thereby raising further concerns over the safety of e-cigarette use. This article has an associated First Person interview with the first author of the paper.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.