ArticleAnalytical chemistry2022
High-Throughput Nano-DESI Mass Spectrometry Imaging of Biological Tissues Using an Integrated Microfluidic Probe.
Article in Analytical chemistry, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 1 synthesis or guideline pooled it, 43 citations in OpenAlex.
- Molecular imaging of bacterial biofilms-a systematic review.Critical reviews in microbiology · 2024Pooled it
- Fabrication of thermoplastic microfluidic probes for nanospray desorption electrospray ionization mass spectrometry.International journal of mass spectrometry · 2026Article
- Imaging strategies for delineating glioma margins: Aiming at precise therapy.Fundamental research · 2026Review
- Ambient high-spatial-resolution mass spectrometry imaging: recent advances in technologies and metabolomics studies.Trends in analytical chemistry : TRAC · 2026Article
- Achieving Single-Cell Resolution via Desorption Electrospray Ionization Mass Spectrometry Imaging (DESI-MSI) on Different Platforms.Analytical chemistry · 2026Article
- High-spatial-resolution mass spectrometry imaging of biological tissues using a microfluidic probe.Nature protocols · 2026Review
- Innovative Application of a Multifunctional Sucrose-Gelatin Hydrogel Matrix in Desorption Electrospray Ionization-Mass Spectrometry Imaging.Analytical chemistry · 2025Article
- Pneumatically Assisted Microfluidic Probe for Enhanced Mass Spectrometry Imaging Performance.Journal of the American Society for Mass Spectrometry · 2025Article
- [Recent progress in mass spectrometry imaging using nanospray desorption electrospray ionization].Se pu = Chinese journal of chromatography · 2025Review
- Nanospray Desorption Electrospray Ionization Mass Spectrometry Imaging (nano-DESI MSI): A Tutorial Review.ACS measurement science au · 2024Review
- MassLite: An integrated python platform for single cell mass spectrometry metabolomics data pretreatment with graphical user interface and advanced peak alignment method.Analytica chimica acta · 2024Article
- Hardware and software solutions for implementing nanospray desorption electrospray ionization (nano-DESI) sources on commercial mass spectrometers.Journal of mass spectrometry : JMS · 2024Article
- Atmospheric Pressure Mass Spectrometry Imaging Using Electrospray-Assisted Laser Desorption/Ionization with Gas Transportation through a Heated Tube and Minimal Sample Preparation.Mass spectrometry (Tokyo, Japan) · 2024Article
- Next Generation of Mass Spectrometry Imaging: from Micrometer to Subcellular Resolution.Chemical & biomedical imaging · 2023Review
- A monolithic microfluidic probe for ambient mass spectrometry imaging of biological tissues.Lab on a chip · 2023Article
- High-resolution integrated microfluidic probe for mass spectrometry imaging of biological tissues.Analytica chimica acta · 2023Article
- Mechanism underlying the effect of MnOMikrochimica acta · 2023Article
- Nanospray Desorption Electrospray Ionization (Nano-DESI) Mass Spectrometry Imaging with High Ion Mobility Resolution.Journal of the American Society for Mass Spectrometry · 2023Article
- Applications of ambient ionization mass spectrometry in 2022: An annual review.Analytical science advances · 2023Review
- High-Throughput Mass Spectrometry Imaging of Biological Systems: Current Approaches and Future Directions.Trends in analytical chemistry : TRAC · 2023Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Nanospray desorption electrospray mass spectrometry imaging (nano-DESI MSI) enables quantitative mapping of hundreds of molecules in biological samples with minimal sample pretreatment. We have recently developed an integrated microfluidic probe (iMFP) for nano-DESI MSI. Herein, we describe an improved design of the iMFP for the high-throughput imaging of tissue sections. We increased the dimensions of the primary and spray channels and optimized the spray voltage and solvent flow rate to obtain a stable operation of the iMFP at both low and high scan rates. We observe that the sensitivity, molecular coverage, and spatial resolution obtained using the iMFP do not change to a significant extent as the scan rate increases. Using a scan rate of 0.4 mm/s, we obtained high-quality images of mouse uterine tissue sections (scan area: 3.2 mm × 2.3 mm) in only 9.5 min and of mouse brain tissue (scan area: 7.0 mm × 5.4 mm) in 21.7 min, which corresponds to a 10-15-fold improvement in the experimental throughput. We have also developed a quantitative metric for evaluating the quality of ion images obtained at different scan rates. Using this metric, we demonstrate that the quality of nano-DESI MSI data does not degrade substantially with an increase in the scan rate. The ability to image biological tissues with high throughput using iMFP-based nano-DESI MSI will substantially speed up tissue mapping efforts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.