Evidence map›Paper›PMID 35769923›Full record

ArticleSTAR protocols2022

Fast and high-fidelity

Mehmet Saçma, Francesca Matteini, Medhanie A Mulaw, Ali Hageb, Ruzhica Bogeska, Vadim Sakk, Angelika Vollmer, Gina Marka, Karin Soller, Michael D Milsom and 2 more

Abstract read
In one paragraph

Article in STAR protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Low-Dose Ionizing γ-Radiation Elicits the Extrusion of Neutrophil Extracellular Traps.Clinical cancer research : an official journal of the American Association for Cancer Research · 2024
    Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Mehmet SaçmaInstitute of Molecular Medicine, Ulm University, 89081 Ulm, Germany. Electronic address: mehmet.sacma@uni-ulm.de.
Francesca MatteiniStem Cell Aging Group, Regenerative Medicine Program, The Bellvitge Institute for Biomedical Research (IDIBELL), L'Hospitalet de Llobregat, Barcelona, Spain; Program for Advancing the Clinical Translation of Regenerative Medicine of Catalonia, P-CMR[C], L'Hospitalet de Llobregat, Barcelona, Spain.
Medhanie A MulawMolecular Oncology Institute of Experimental Cancer Research, Medical Faculty, Ulm University, Ulm, Germany.
Ali HagebInstitute of Molecular Medicine, Ulm University, 89081 Ulm, Germany.
Ruzhica BogeskaDivision of Experimental Hematology, German Cancer Research Center (DKFZ) and Heidelberg Institute for Stem Cell Technology and Experimental Medicine (HI-STEM), Heidelberg, Germany.
Vadim SakkInstitute of Molecular Medicine, Ulm University, 89081 Ulm, Germany.
Angelika VollmerInstitute of Molecular Medicine, Ulm University, 89081 Ulm, Germany.
Gina MarkaInstitute of Molecular Medicine, Ulm University, 89081 Ulm, Germany.
Karin SollerInstitute of Molecular Medicine, Ulm University, 89081 Ulm, Germany.
Michael D MilsomDivision of Experimental Hematology, German Cancer Research Center (DKFZ) and Heidelberg Institute for Stem Cell Technology and Experimental Medicine (HI-STEM), Heidelberg, Germany.
Maria Carolina FlorianStem Cell Aging Group, Regenerative Medicine Program, The Bellvitge Institute for Biomedical Research (IDIBELL), L'Hospitalet de Llobregat, Barcelona, Spain; Program for Advancing the Clinical Translation of Regenerative Medicine of Catalonia, P-CMR[C], L'Hospitalet de Llobregat, Barcelona, Spain; Center for Networked Biomedical Research on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid, Spain.
Hartmut GeigerInstitute of Molecular Medicine, Ulm University, 89081 Ulm, Germany. Electronic address: hartmut.geiger@uni-ilm.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Quantitative 3D imaging of organ-wide cellular and subcellular components is central for revealing and understanding complex interactions between stem cells and their microenvironment. Here, we present a gentle but fast whole-mount immunofluorescence staining protocol for 3D confocal microscopy (iFAST3D) that preserves the 3D structure of the entire tissue and that of subcellular structures with high fidelity. The iFAST3D protocol enables reproducible and high-resolution 3D imaging of stem cells and various niche components for many mouse organs and tissues. For complete details on the use and execution of this protocol, please refer to Saçma et al. (2019).

Indexed as

Imaging, Three-DimensionalStem CellsAnimalsMiceMicroscopy, ConfocalStaining and LabelingCancerCell BiologyMicroscopyStem Cells

Identifiers

PMID35769923
PMCPMC9234157

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.