ReviewNature protocols2022
A practical guide to scanning light-field microscopy with digital adaptive optics.
Review in Nature protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 29 citations in OpenAlex.
- High numerical aperture confocal volumetric mesoscope reveals mesoscale subcellular dynamics in vivo.Nature biotechnology · 2026Article
- Real-time robust autofocus method enabling sustained intravital scanning light field imaging.Nature communications · 2026Article
- Intravital mesoscale optical imaging: challenges, techniques, and future perspectives.Biophysics reports · 2026Article
- Snapshot 3D at the speed frontier: redefining light-field microscopy for neuroimaging.PhotoniX · 2026Article
- Leveraging spatial-angular redundancy for self-supervised denoising of 3D fluorescence imaging without temporal dependency.Nature communications · 2025Article
- Gut-derived indole propionic acid alleviates liver fibrosis by targeting profibrogenic macrophages via the gut‒liver axis.Cellular & molecular immunology · 2025Article
- Non-invasive large-scale imaging of concurrent neuronal, astrocytic, and hemodynamic activity with hybrid multiplexed fluorescence and magnetic resonance imaging (HyFMRI).Light, science & applications · 2025Article
- Physics-driven self-supervised learning for fast high-resolution robust 3D reconstruction of light-field microscopy.Nature methods · 2025Article
- High-speed fluorescence lifetime imaging microscopy: techniques, applications, and prospects.Biophotonics discovery · 2025Review
- Real-time and universal network for volumetric imaging from microscale to macroscale at high resolution.Light, science & applications · 2025Article
- Long-term intravital subcellular imaging with confocal scanning light-field microscopy.Nature biotechnology · 2025Article
- High-fidelity and fast 3D imaging of subcellular dynamics in native states.Nature biotechnology · 2025Article
- Ultrafast optical imaging techniques for exploring rapid neuronal dynamics.Neurophotonics · 2025Review
- GPR116 alleviates acetaminophen-induced liver injury in mice by inhibiting endoplasmic reticulum stress.Cellular and molecular life sciences : CMLS · 2024Article
- Efficient high-resolution fluorescence projection imaging over an extended depth of field through optical hardware and deep learning optimizations.Biomedical optics express · 2024Article
- Myeloid-Mas Signaling Modulates Pathogenic Crosstalk among MYCAdvanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Virtual-scanning light-field microscopy for robust snapshot high-resolution volumetric imaging.Nature methods · 2023Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
With the development of a wide variety of animal models in recent years, there is a rapidly growing demand for long-term, high-speed intravital fluorescence imaging to observe intercellular and intracellular interactions in their native states. Scanning light-field microscopy (sLFM) with digital adaptive optics provides a compact computational solution by imaging the entire volume in a tomographic way with orders-of-magnitude improvement in spatiotemporal resolution and reduction in phototoxicity, as compared to traditional intravital microscopy. Here, we present a step-by-step protocol for both hardware and software implementation of multicolor sLFM as an add-on to a normal wide-field fluorescence microscope by using off-the-shelf lenses and devices at low cost. The procedure can be easily applied to other LFM variants, which can be advantageous in certain experimental contexts. Owing to the strong reliance of sLFM on algorithmic post-processing for high-quality data, the protocol describes various kinds of artefacts and corresponding parameters used for correcting and performance optimization. To increase the tolerance to system misalignment and differences in device fabrication, we describe a one-step calibration method for robust imaging performance up to the diffraction limit. An open-source graphical user interface is presented for hardware synchronization and real-time rendering of multiview images. The whole procedure including optical setup, software installation, system calibration and 3D reconstruction can be executed in 3-4 d with basic knowledge in optics and electronics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.