ArticlePLoS genetics2022
Two duplicated gsdf homeologs cooperatively regulate male differentiation by inhibiting cyp19a1a transcription in a hexaploid fish.
Article in PLoS genetics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 26 citations in OpenAlex.
- Full-Length Transcriptome Comparison of Male and Female Gonads inBiomolecules · 2026Article
- Integrated Transcriptome Landscape of mRNAs, lncRNAs, circRNAs, and miRNAs Reveals Molecular Regulatory Networks of Sex Differentiation in the Zig-Zag Eel (International journal of molecular sciences · 2026Article
- Characterization of Forkhead Box Transcription Factor (Animals : an open access journal from MDPI · 2026Article
- Comparative RNA-Seq Analysis of Differentially Expressed Genes in the Testis and Ovary of Mudskipper,Animals : an open access journal from MDPI · 2026Article
- Sex- and stage-dependent expression of gonadal soma-derived factor paralogues reveals functional and evolutionary divergence in European sea bass (Frontiers in endocrinology · 2026Article
- amh alleles on XY sex chromosomes regulate male differentiation in amphidiploid Carassius auratus.Science China. Life sciences · 2026Article
- Role of multi-omics in aquaculture genetics and breeding: current status and future perspective.Science China. Life sciences · 2025Review
- Anti-Müllerian hormone signalling sustains circadian homeostasis in zebrafish.Nature communications · 2025Article
- Establishment, identification, and transcriptome analysis of a Sertoli cell line from ovoviviparous black rockfish Sebastes schlegelii.Fish physiology and biochemistry · 2025Article
- Decoding the fish genome opens a new era in important trait research and molecular breeding in China.Science China. Life sciences · 2024Review
- Genomic Anatomy of Homozygous XX Females and YY Males Reveals Early Evolutionary Trajectory of Sex-determining Gene and Sex Chromosomes in Silurus Fishes.Molecular biology and evolution · 2024Article
- Dmrt1 is the only male pathway gene tested indispensable for sex determination and functional testis development in tilapia.PLoS genetics · 2024Article
- Causal gene identification and desirable trait recreation in goldfish.Science China. Life sciences · 2022Article
- Changes in Ploidy Drive Reproduction Transition and Genomic Diversity in a Polyploid Fish Complex.Molecular biology and evolution · 2022Article
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Authors and funding
16 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Although evolutionary fates and expression patterns of duplicated genes have been extensively investigated, how duplicated genes co-regulate a biological process in polyploids remains largely unknown. Here, we identified two gsdf (gonadal somatic cell-derived factor) homeologous genes (gsdf-A and gsdf-B) in hexaploid gibel carp (Carassius gibelio), wherein each homeolog contained three highly conserved alleles. Interestingly, gsdf-A and gsdf-B transcription were mainly activated by dmrt1-A (dsx- and mab-3-related transcription factor 1) and dmrt1-B, respectively. Loss of either gsdf-A or gsdf-B alone resulted in partial male-to-female sex reversal and loss of both caused complete sex reversal, which could be rescued by a nonsteroidal aromatase inhibitor. Compensatory expression of gsdf-A and gsdf-B was observed in gsdf-B and gsdf-A mutants, respectively. Subsequently, we determined that in tissue culture cells, Gsdf-A and Gsdf-B both interacted with Ncoa5 (nuclear receptor coactivator 5) and blocked Ncoa5 interaction with Rora (retinoic acid-related orphan receptor-alpha) to repress Rora/Ncoa5-induced activation of cyp19a1a (cytochrome P450, family 19, subfamily A, polypeptide 1a). These findings illustrate that Gsdf-A and Gsdf-B can regulate male differentiation by inhibiting cyp19a1a transcription in hexaploid gibel carp and also reveal that Gsdf-A and Gsdf-B can interact with Ncoa5 to suppress cyp19a1a transcription in vitro. This study provides a typical case of cooperative mechanism of duplicated genes in polyploids and also sheds light on the conserved evolution of sex differentiation.
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