Evidence map›Paper›PMID 35767115›Full record

ArticleCurrent microbiology2022

Rep-PCR Analyses Reveal Genetic Variation of Ralstonia solanacearum Causing Wilt of Solanaceaous Vegetables in Bangladesh.

Md Mosharraf Hossain, Md Mostafa Masud, Muhammad Iqbal Hossain, Mohammad Mahbubul Haque, Mohammad Sharif Uddin, Md Zahangir Alam, Md Rashidul Islam

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Article in Current microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

7 authors.

Md Mosharraf HossainAgricultural Research Station (ARS), Bangladesh Agricultural Research Institute (BARI), Satkhira, 9403, Bangladesh.
Md Mostafa MasudPlant Bacteriology and Biotechnology Laboratory, Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh.
Muhammad Iqbal HossainPlant Bacteriology and Biotechnology Laboratory, Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh.
Mohammad Mahbubul HaquePlant Pathology Division, Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh, 2202, Bangladesh.
Mohammad Sharif UddinDepartment of Microbiology, Noakhali Science and Technology University, Noakhali, 3814, Bangladesh.
Md Zahangir AlamPlant Bacteriology and Biotechnology Laboratory, Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh.
Md Rashidul IslamPlant Bacteriology and Biotechnology Laboratory, Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh. rashidul.islam@bau.edu.bd.ORCID http://orcid.org/0000-0002-0790-6521

Funding

The Academy of Sciences for the Developing World 14-019 RG/BIO/AS_I
6 · The paper itself

Abstract

Ralstonia solanacearum, a soil-borne and seed-borne plant pathogenic bacterium, causes bacterial wilt to several important crop plants causing substantial economic losses. To provide population information on this pathogen for developing effective control strategies, Rep-PCR was used to analyze the genetic variation of 18 representative isolates of R. solanacearum collected in Bangladesh. Phenotypic analyses revealed that all eighteen isolates belong to biotype 3 with wide diversity in aggressiveness on eggplant, tomato, and chili. Rep-PCR studies utilizing the REP, ERIC, and BOXIR primers showed a wide variation at the genetic level among the R. solanacearum isolates used in this study. Dendrogram constructed using REP, ERIC, and BOXIR primers based on banding patterns implied that R. solanacearum isolates were genetically diversified and distributed in four clusters at 83%, 80%, and 63% similarity index, respectively. The genetic relationship assayed by rep-PCR highlighted a wide range of genetic variation but no relation among geographical origin, aggressiveness, and phylogenetic groups of R. solanacearum isolates. These results conceded that other molecular markers related to virulence gene(s) might reveal the complex relationship among geographical origin, aggressiveness, and phylogenetic groups.

Indexed as

Ralstonia solanacearumSolanum lycopersicumBangladeshDNA PrimersGenetic VariationPhylogenyPlant DiseasesPlantsPolymerase Chain ReactionVegetablesDNA Primers

Identifiers

PMID35767115

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