Evidence map›Paper›PMID 35767109›Full record

ArticleMolecular biology reports2022

Cloning and screening of the putative hexokinase genes from Rhizopus oryzae and their heterologous expression in Saccharomyces cerevisiae.

Eda Alagöz, Şeyda Açar, Meral Yücel, Haluk Hamamcı

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Article in Molecular biology reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.2field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Eda AlagözDepartment of Biotechnology, Middle East Technical University, Ankara, Turkey. alagozeda@yahoo.com.ORCID http://orcid.org/0000-0002-6670-5623
Şeyda AçarDepartment of Biotechnology, Middle East Technical University, Ankara, Turkey.
Meral YücelDepartment of Biotechnology, Middle East Technical University, Ankara, Turkey.
Haluk HamamcıDepartment of Biotechnology, Middle East Technical University, Ankara, Turkey.
Middle East Technical University · TR

Funding

Türkiye Bilimsel ve Teknolojik Araştirma Kurumu 112T662
6 · The paper itself

Abstract

backgroundA filamentous fungus, Rhizopus oryzae (R. oryzae) is one of the ideal candidates for ethanol and lactic acid production due to its ability to grow on renewable carbon sources. METHODS AND

resultsIn this study, the nucleotide sequence of hexokinases and glucokinase from S. cerevisiae was found on the NCBI site ( http://www.ncbi.nlm.nih.gov/blast/Blast.cgi ) were used. With these nucleotide sequences, a blast search was done on the R. oryzae genome database ( http://www.broad.mit.edu/annotation/genome/rhizopus_oryzae/Home.html ) and ten probable genes were obtained. cDNA was synthesized from the total RNA and PCR products of the seven of these putative genes were determined using the primers designed for them.

conclusionThe results of the sequences and the complementation studies revealed that three of these seven putative genes were expressed in R. oryzae and the growth was observed on selective media.

Indexed as

HexokinaseSaccharomyces cerevisiaeCloning, MolecularRhizopusRhizopus oryzaeHexokinaseCloningHexokinaseLactic acidRhizopus oryzaeSaccharomyces cerevisiaeTransformation

Identifiers

PMID35767109
OpenAlexW4283713070

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.