Evidence map›Paper›PMID 35765657›Full record

ArticleJournal of life sciences (Westlake Village, Calif.)2021

A timeseries analysis of the fracture callus extracellular matrix proteome during bone fracture healing.

Christopher B Erickson, Ryan Hill, Donna Pascablo, Galateia Kazakia, Kirk Hansen, Chelsea Bahney

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of life sciences (Westlake Village, Calif.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed, 1 pooled it
0.4field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 1 synthesis or guideline pooled it, 4 citations in OpenAlex.

  1. Pooled it
  2. Review
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Christopher B EricksonDepartment of Biochemistry and Molecular Genetics,University of Colorado, Anschutz Medical Campus, Aurora, CO.
Ryan HillDepartment of Biochemistry and Molecular Genetics,University of Colorado, Anschutz Medical Campus, Aurora, CO.
Donna PascabloOrthopaedic Trauma Institute, University of California, San Francisco (UCSF), San Francisco, CA.
Galateia KazakiaDepartment of Radiology and Biomedical Imaging, University of California, San Francisco (UCSF), San Francisco, CA.
Kirk HansenDepartment of Biochemistry and Molecular Genetics,University of Colorado, Anschutz Medical Campus, Aurora, CO.
Chelsea BahneyStedman Philippon Research Institute (SPRI), Center for Regenerative and Personalized Medicine. Vail, CO.
University of California, San Francisco · US

Funding

Engaging University of California Stakeholders for Biorespository ResearchUL1TR000004 · NCATS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI GRANDIS, JENNIFER RUBIN · 2012 to 2015
$78.0M
Therapeutic Application of Painless Nerve Growth Factor to Accelerate Endochondral Fracture RepairR01AR077761 · NIAMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI BAHNEY, CHELSEA SHIELDS · 2021 to 2025
$3.2M
NCATS NIH HHS UL1 TR000004NIAMS NIH HHS R01 AR077761
6 · The paper itself

Abstract

While most bones fully self-heal, certain diseases require bone allograft to assist with fracture healing. Bone allografts offer promise as treatments for such fractures due to their osteogenic properties. However, current bone allografts made of decellularized bone extracellular matrix (ECM) have high failure rates, and thus grafts which improve fracture healing outcomes are needed. Understanding specific changes to the ECM proteome during normal fracture healing would enable the identification of key proteins that could be used enhance osteogenicity of bone allograft. Here, we performed a timeseries analysis of the fracture callus in mice to investigate proteomic and mineralization changes to the ECM at key stages of fracture healing. We found that changes to the ECM proteome largely coincide with the distinct phases of fracture healing. Basement membrane proteins (AGRN, COL4, LAMA), cartilage proteins (COL2A1, ACAN), and collagen crosslinking enzymes (LOXL, PLOD, ITIH) were initially upregulated, followed by bone specific proteoglycans and collagens (IBSP, COL1A1). Various tissue proteases (MMP2, 9, 13, 14; CTSK, CTSG, ELANE) were expressed at different levels throughout fracture healing. These changes coordinated with mineralization of the fracture callus, which increased steeply during the initial stages of healing. Interestingly the later timepoint was characterized by a response to wound healing and high expression of clotting factors (F2, 7, 9, 10). We identified ELANE and ITIH2 as tissue remodeling enzymes having no prior known involvement with fracture healing. This data can be further mined to identify regenerative proteins for enhanced bone graft design.

Identifiers

PMID35765657
PMCPMC9236279
OpenAlexW4281737229

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.