ArticlePlant methods2022
Generation of the salicylic acid deficient Arabidopsis via a synthetic salicylic acid hydroxylase expression cassette.
Article in Plant methods, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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4 citing papers in PubMed, 7 citations in OpenAlex.
- AtMPK1/AtMPK2 Interact with AtVQ25 to Enhance Its Protein Stability and Coordinately Regulate Salicylic Acid-Mediated Leaf Senescence.Plants (Basel, Switzerland) · 2026Article
- Salicylic acid and the unique TGA transcription factor controls plant immunity against Pseudomonas syringae in Marchantia polymorpha.Plant & cell physiology · 2026Article
- Comprehensive genomic identification of essential genes required for Brucella melitensis intracellular survival during macrophage infection.Scientific reports · 2026Article
- Exploring the genetic landscape of sesame: whole genome sequencing reveals distinct Egyptian lineages and SNP variability.Molecular genetics and genomics : MGG · 2026Article
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10 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundSalicylic acid (SA) is one of the plant hormones, which plays crucial roles in signaling transduction in plant growth, disease resistance, and leaf senescence. Arabidopsis (Arabidopsis thaliana) SA 3-hydroxylase (S3H) and 5-hydroxylase (S5H) are key enzymes which maintain SA homeostasis by catalyzing SA to 2,3-dihydroxybenzoic acid (DHBA) and 2,5-DHBA, respectively.
resultsSA deficient transgenic Arabidopsis lines were generated by introducing two binary vectors S5Hpro::EGFP-S3H and 35Spro::EGFP-S3H respectively, in which the expression of S3H is under the control of the S5H promoter or CaMV 35S promoter. Compared with the constitutive expression of S3H gene under the control of 35S promoter, the S3H gene under the native S5H promoter is activated by endogenous SA and results in a dynamic control of SA catabolism in a feedback mode. The SA accumulation, growth, leaf senescence, and pathogen resistance of the S5Hpro::GFP-S3H transgenic plants were investigated in parallel with NahG transgenic plants. The SA levels in the S5Hpro::EGFP-S3H transgenic plants were similar to or slightly lower than those of NahG transgenic Arabidopsis and resulted in SA deficient phenotypes. The low-SA trait of the S5Hpro::EGFP-S3H transgenic lines was inherited stably in the later generations.
conclusionsCompared with NahG transgenic lines producing by-product catechol, S5Hpro::EGFP-S3H transgenic lines reduce SA levels by converting SA to a native product 2,3-DHBA for catabolism. Together, we provide new SA-deficient germplasms for the investigations of SA signaling in plant development, leaf senescence, and disease resistance.
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