ArticleJournal of biological engineering2022
Cloning, expression, and one-step purification/immobilization of two carbohydrate-binding module-tagged alcohol dehydrogenases.
Article in Journal of biological engineering, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 16 citations in OpenAlex.
- Immobilized Hydroxymethylfurfural Oxidase Enables Robust Biocatalytic Production of 2,5-Furandicarboxylic Acid from Crude 5‑Hydroxymethylfurfural.ACS sustainable chemistry & engineering · 2026Article
- Enzyme-Powered COACS sustainable chemistry & engineering · 2026Article
- Advances and Challenges in the Development of Immobilized Enzymes for Batch and Flow Biocatalyzed Processes.ChemSusChem · 2025Review
- Lactic Acid from COEnvironmental science & technology · 2023Article
- Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe feasibility of biochemical transformation processes is usually greatly dependent on biocatalysts cost. Therefore, immobilizing and reusing biocatalysts is an approach to be considered to bring biotransformations closer to industrial feasibility, since it does not only allow to reuse enzymes but can also improve their stability towards several reaction conditions. Carbohydrate-Binding Modules (CBM) are well-described domains involved in substrate binding which have been already used as purification tags.
resultsIn this work, two different Carbohydrate-Binding Modules (CBM3 and CBM9) have been successfully fused to an alcohol dehydrogenase from Saccharomyces cerevisiae, which has been produced in bench-scale reactor using an auxotrophic M15-derived E. coli strain, following a fed-batch strategy with antibiotic-free medium. Around 40 mg·g
conclusionCBM domains were proved to be suitable for one-step immobilization/purification process, retaining almost total activity offered. However, purification process was only successful with CBM9.
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