ArticleJournal of reconstructive microsurgery2023
Exceeding the Limits of Static Cold Storage in Limb Transplantation Using Subnormothermic Machine Perfusion.
Article in Journal of reconstructive microsurgery, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 2 syntheses or guidelines pooled it, 36 citations in OpenAlex.
- Transplant international : official journal of the European Society for Organ Transplantation · 2025Pooled it
- A meta-analysis of perfusion parameters affecting weight gain in ex vivo perfusion.Artificial organs · 2025Pooled it
- Acellular Normothermic Machine Perfusion Does not Exacerbate Acute Rejection in Rodent Vascularized Composite Allografts.Transplantation direct · 2026Article
- Development of a Swine Whole Eye Transplant Ex Vivo Perfusion Protocol.Artificial organs · 2026Article
- Small animalWorld journal of gastroenterology · 2026Review
- Systematic review of emerging technologies in vascularized composite allotransplantation.Frontiers in transplantation · 2026Review
- 24-hourFrontiers in transplantation · 2026Article
- Development of a 24-h Preservation Protocol of Forearm Vascularized Composite Allotransplants in Nonhuman Primates Using Subnormothermic Machine Perfusion.Transplantation direct · 2025Article
- Vascular Microphysiological System for Investigating Endothelial Barrier Function During Organ Preservation and Reperfusion.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- [Advances and perspectives in vascularized composite allotransplantation preservation].Bulletin de l'Academie nationale de medecine · 2024Article
- A Guide to the Implementation and Design of Ex Vivo Perfusion Machines for Vascularized Composite Allotransplantation.Plastic and reconstructive surgery. Global open · 2024Article
- Enhanced VCA Storage: A Pilot Study Demonstrating Supercooling in Orthotopic Rodent Hindlimb Transplantation.Transplantation proceedings · 2024Article
- Supercooling preservation of vascularized composite allografts through CPA optimization, thermal tracking, and stepwise loading techniques.Scientific reports · 2024Article
- Sub-zero non-freezing of vascularized composite allografts in a rodent partial hindlimb model.Cryobiology · 2024Article
- VCA supercooling in a swine partial hindlimb model.Scientific reports · 2024Article
- Successful Extension of Vascularized Composite Allograft Perfusion Cold Storage to 24 h in a Rat Hindlimb Transplant Model.Transplantation direct · 2024Article
- Study on the preservation effects of the amputated forelimb by machine perfusion at physiological temperature.Chinese journal of traumatology = Zhonghua chuang shang za zhi · 2024Article
- Focusing on Ischemic Reperfusion Injury in the New Era of Dynamic Machine Perfusion in Liver Transplantation.International journal of molecular sciences · 2024Review
- Machine Perfusion Enables 24-h Preservation of Vascularized Composite Allografts in a Swine Model of Allotransplantation.Transplant international : official journal of the European Society for Organ Transplantation · 2024Article
- Towards Optimizing Sub-Normothermic Machine Perfusion in Fasciocutaneous Flaps: A Large Animal Study.Bioengineering (Basel, Switzerland) · 2023Article
Corrections and comments
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Authors and funding
12 authors at 5 institutions in 3 countries.
Funding
Abstract
backgroundFor 50 years, static cold storage (SCS) has been the gold standard for solid organ preservation in transplantation. Although logistically convenient, this preservation method presents important constraints in terms of duration and cold ischemia-induced lesions. We aimed to develop a machine perfusion (MP) protocol for recovery of vascularized composite allografts (VCA) after static cold preservation and determine its effects in a rat limb transplantation model.
methodsPartial hindlimbs were procured from Lewis rats and subjected to SCS in Histidine-Tryptophan-Ketoglutarate solution for 0, 12, 18, 24, and 48 hours. They were then either transplanted (Txp), subjected to subnormothermic machine perfusion (SNMP) for 3 hours with a modified Steen solution, or to SNMP + Txp. Perfusion parameters were assessed for blood gas and electrolytes measurement, and flow rate and arterial pressures were monitored continuously. Histology was assessed at the end of perfusion. For select SCS durations, graft survival and clinical outcomes after transplantation were compared between groups at 21 days.
resultsTransplantation of limbs preserved for 0, 12, 18, and 24-hour SCS resulted in similar survival rates at postoperative day 21. Grafts cold-stored for 48 hours presented delayed graft failure (
conclusionTo date, VCA MP is still limited to animal models and no protocols are yet developed for graft recovery. Our study suggests that ex vivo SNMP could help increase the preservation duration and limit cold ischemia-induced injury in VCA transplantation.
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