Evidence map›Paper›PMID 35763352›Full record

ArticleMolecular ecology resources2022

Transcript- and annotation-guided genome assembly of the European starling.

Katarina C Stuart, Richard J Edwards, Yuanyuan Cheng, Wesley C Warren, David W Burt, William B Sherwin, Natalie R Hofmeister, Scott J Werner, Gregory F Ball, Melissa Bateson and 7 more

Abstract read
In one paragraph

Article in Molecular ecology resources, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed.

  1. Genome assembly and insights into globally invasive Red-vented Bulbul (Pycnonotus cafer).DNA research : an international journal for rapid publication of reports on genes and genomes · 2025
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  7. The genome of a globally invasive passerine, the common myna, Acridotheres tristis.DNA research : an international journal for rapid publication of reports on genes and genomes · 2024
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Katarina C StuartEvolution & Ecology Research Centre, School of Biological, Earth and Environmental Sciences, UNSW Sydney, Sydney, New South Wales, Australia.ORCID https://orcid.org/0000-0002-0386-4600
Richard J EdwardsEvolution & Ecology Research Centre, School of Biotechnology and Biomolecular Sciences, UNSW Sydney, Sydney, New South Wales, Australia.ORCID https://orcid.org/0000-0002-3645-5539
Yuanyuan ChengSchool of Life and Environmental Sciences, The University of Sydney, Sydney, New South Wales, Australia.ORCID https://orcid.org/0000-0002-1747-9308
Wesley C WarrenDepartment of Animal Sciences, Institute for Data Science and Informatics, The University of Missouri, Columbia, Missouri, USA.
David W BurtOffice of the Deputy Vice-Chancellor (Research and Innovation), The University of Queensland, Brisbane, Australia.ORCID https://orcid.org/0000-0002-9991-1028
William B SherwinEvolution & Ecology Research Centre, School of Biological, Earth and Environmental Sciences, UNSW Sydney, Sydney, New South Wales, Australia.ORCID https://orcid.org/0000-0002-1578-8473
Natalie R HofmeisterDepartment of Ecology and Evolutionary Biology, Cornell University, New York, USA.ORCID https://orcid.org/0000-0002-6875-412X
Scott J WernerUnited States Department of Agriculture, Animal and Plant Health Inspection Service, Wildlife Services, National Wildlife Research Center, Fort Collins, Colorado, USA.ORCID https://orcid.org/0000-0002-3483-7402
Gregory F BallDepartment of Psychology, University of Maryland, Maryland, USA.ORCID https://orcid.org/0000-0002-4784-0520
Melissa BatesonInstitute of Neuroscience, Newcastle University, Newcastle upon Tyne, UK.ORCID https://orcid.org/0000-0002-0861-0191
Matthew C BrandleySection of Amphibians and Reptiles, Carnegie Museum of Natural History, Pittsburgh, Pennsylvania, USA.ORCID https://orcid.org/0000-0002-3734-913X
Katherine L BuchananSchool of Life and Environmental Sciences, Deakin University, Waurn Ponds, Victoria, Australia.ORCID https://orcid.org/0000-0002-6648-5819
Phillip CasseyInvasion Science & Wildlife Ecology Lab, University of Adelaide, Adelaide, Australia.ORCID https://orcid.org/0000-0002-2626-0172
David F ClaytonDepartment of Genetics & Biochemistry, Clemson University, South Carolina, USA.ORCID https://orcid.org/0000-0002-6395-3488
Tim De MeyerDepartment of Data Analysis & Mathematical Modelling, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium.ORCID https://orcid.org/0000-0003-2994-9693
Simone L MeddleThe Roslin Institute, The Royal (Dick) School of Veterinary Studies, The University of Edinburgh, Midlothian, UK.ORCID https://orcid.org/0000-0002-5629-1332
Lee A RollinsEvolution & Ecology Research Centre, School of Biological, Earth and Environmental Sciences, UNSW Sydney, Sydney, New South Wales, Australia.ORCID https://orcid.org/0000-0002-3279-7005

Funding

Australian Research Council LP160100610Australian Research Council LP18010072Biotechnology and Biological Sciences Research Council P013759Human Sciences Frontier Programme RGP0030/2015Roslin Institute Strategic Grant funding from the UK Biotechnology and Biological Sciences Research Council BB/P013759/1University of New South Wales Scientia Fellowship
6 · The paper itself

Abstract

The European starling, Sturnus vulgaris, is an ecologically significant, globally invasive avian species that is also suffering from a major decline in its native range. Here, we present the genome assembly and long-read transcriptome of an Australian-sourced European starling (S. vulgaris vAU), and a second, North American, short-read genome assembly (S. vulgaris vNA), as complementary reference genomes for population genetic and evolutionary characterization. S. vulgaris vAU combined 10× genomics linked-reads, low-coverage Nanopore sequencing, and PacBio Iso-Seq full-length transcript scaffolding to generate a 1050 Mb assembly on 6222 scaffolds (7.6 Mb scaffold N50, 94.6% busco completeness). Further scaffolding against the high-quality zebra finch (Taeniopygia guttata) genome assigned 98.6% of the assembly to 32 putative nuclear chromosome scaffolds. Species-specific transcript mapping and gene annotation revealed good gene-level assembly and high functional completeness. Using S. vulgaris vAU, we demonstrate how the multifunctional use of PacBio Iso-Seq transcript data and complementary homology-based annotation of sequential assembly steps (assessed using a new tool, saaga) can be used to assess, inform, and validate assembly workflow decisions. We also highlight some counterintuitive behaviour in traditional busco metrics, and present buscomp, a complementary tool for assembly comparison designed to be robust to differences in assembly size and base-calling quality. This work expands our knowledge of avian genomes and the available toolkit for assessing and improving genome quality. The new genomic resources presented will facilitate further global genomic and transcriptomic analysis on this ecologically important species.

Indexed as

StarlingsAnimalsAustraliaGenomeGenomicsMolecular Sequence Annotationfull-length transcriptsgenome annotationgenome assemblygenome assessmentSturnus vulgaris

Identifiers

PMID35763352
PMCPMC9796300

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.