Evidence map›Paper›PMID 35763180›Full record

ArticleMolecular biology reports2022

Cordycepin enhances hyperthermia-induced apoptosis and cell cycle arrest by modulating the MAPK pathway in human lymphoma U937 cells.

Liying Shi, He Cao, Siyu Fu, Zixian Jia, Xuan Lu, Zhengguo Cui, Dayong Yu

Abstract read
PubMed Publisher
In one paragraph

Article in Molecular biology reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.6field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 9 citations in OpenAlex.

  1. Review
  2. Review
  3. The role and mechanisms of cordycepin in inhibiting cancer cells.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2024
    Review
  4. Article
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 2 countries.

Liying ShiThe School of Life Science and Biotechnology, Dalian University, Dalian, 116622, People's Republic of China.
He CaoThe School of Life Science and Biotechnology, Dalian University, Dalian, 116622, People's Republic of China.
Siyu FuThe School of Life Science and Biotechnology, Dalian University, Dalian, 116622, People's Republic of China.
Zixian JiaThe School of Life Science and Biotechnology, Dalian University, Dalian, 116622, People's Republic of China.
Xuan LuThe School of Life Science and Biotechnology, Dalian University, Dalian, 116622, People's Republic of China.
Zhengguo CuiDepartment of Environmental Health, University of Fukui School of Medical Science, University of Fukui, Eiheiji, 910-1193, Japan. sai@u-fukui.ac.jp.
Dayong YuThe School of Life Science and Biotechnology, Dalian University, Dalian, 116622, People's Republic of China. yudayong@dlu.edu.cn.ORCID http://orcid.org/0000-0003-4770-4120
Dalian University · CNUniversity of Fukui · JP

Funding

Natural Science Foundation of Liaoning Province 2019-ZD-0564
6 · The paper itself

Abstract

backgroundHyperthermia induces cancer cell death. However, the cytotoxic effect of hyperthermia is not sufficient. Cordycepin can also induce apoptosis in cancer cells and enhance the antitumoral activity of irradiation. To examine cordycepin-mediated enhancement of hyperthermia-induced apoptosis, this study investigated the combined effects and apoptotic mechanisms of hyperthermia and cordycepin on human leukemia U937 cells.

methodsCell viability and apoptosis were measured using MTT assays, Hoechst 33342 staining and Annexin V/PI double staining. The distribution of the cell cycle and sub-G1 phase, reactive oxygen species (ROS) and mitochondrial membrane potential (MMP) were examined by flow cytometry. The expression of related proteins was analyzed by western blotting.

resultsCombined treatment with hyperthermia and cordycepin markedly augmented apoptosis by upregulating Bax and suppressing Bcl-2, Bid and activated caspase 3 and 8 expression, and apoptosis was decreased by Z-VAD-fmk (a pan caspase inhibitor). We also found that the MMP was significantly decreased and excessive ROS generation occurred. The combination treatment also induced arrest in the G2/M phase by downregulating cyclin dependent kinase 1 (CDK1) and cyclin B1 protein expression. Furthermore, it was observed that mitogen-activated protein kinase (MAPK) pathway including ERK, JNK and p38 signals was involved in the induction of apoptosis. The phosphorylated p38 and JNK were increased and ERK phosphorylation was decreased by the combined treatment. In addition, N-acetyl-L-cysteine (NAC) significantly protected the cells by restoring ROS levels and the activity of caspase-3, inactivating the MAPK pathway.

conclusionCordycepin significantly enhanced hyperthermia-induced apoptosis and G2/M phase arrest in U937 cells. The combined treatment enhanced apoptosis through the MAPK pathway and mitochondrial dysfunction, and these effects could be rescued by NAC. We report for the first time that cordycepin can be used as a hyperthermia sensitizer to treat leukemia.

Indexed as

Hyperthermia, InducedLeukemiaLymphomaApoptosisCell Cycle CheckpointsCell Line, TumorDeoxyadenosinesHumansMitogen-Activated Protein Kinasesp38 Mitogen-Activated Protein KinasesReactive Oxygen SpeciesU937 CellscordycepinDeoxyadenosinesMitogen-Activated Protein Kinasesp38 Mitogen-Activated Protein KinasesReactive Oxygen SpeciesApoptosisCell cycleCordycepinHyperthermiaMAPK

Identifiers

PMID35763180
OpenAlexW4283655853

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.