Evidence map›Paper›PMID 35763164›Full record

ArticleBiotechnology letters2022

Enhanced activity of hyperthermostable Pyrococcus horikoshii endoglucanase in superbase ionic liquids.

Hakim Hebal, Joonas Hämäläinen, Laura Makkonen, Alistair W T King, Ilkka Kilpeläinen, Sandip Bankar, Nawel Boucherba, Ossi Turunen

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biotechnology letters, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 54% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 5 institutions in 3 countries.

Hakim HebalLaboratoire de Microbiologie Appliquée (LMA), Faculté des Sciences de La Nature et de La Vie (FSNV), Université de Bejaia, Bejaia, Algeria.
Joonas Hämäläinen, St1 Oy, Firdonkatu 2, PL 68, 00521, Helsinki, Finland.
Laura MakkonenDepartment of Bioproducts and Biosystems, School of Chemical Engineering, Aalto University, 00076, Aalto, Finland.
Alistair W T KingDepartment of Chemistry, University of Helsinki, 00014, Helsinki, Finland.
Ilkka KilpeläinenDepartment of Chemistry, University of Helsinki, 00014, Helsinki, Finland.
Sandip BankarDepartment of Bioproducts and Biosystems, School of Chemical Engineering, Aalto University, 00076, Aalto, Finland.
Nawel BoucherbaLaboratoire de Microbiologie Appliquée (LMA), Faculté des Sciences de La Nature et de La Vie (FSNV), Université de Bejaia, Bejaia, Algeria.
Ossi TurunenSchool of Forest Sciences, University of Eastern Finland, 80101, Joensuu, Finland. ossi.turunen@uef.fi.ORCID http://orcid.org/0000-0002-2593-7436
Aalto University · FIUniversity of Béjaïa · DZUniversity of Helsinki · FINew York City Fire Department · USUniversity of Eastern Finland · FI

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectivesIonic liquids (ILs) that dissolve biomass are harmful to the enzymes that degrade lignocellulose. Enzyme hyperthermostability promotes a tolerance to ILs. Therefore, the limits of hyperthemophilic Pyrococcus horikoschii endoglucanase (PhEG) to tolerate 11 superbase ILs were explored.

resultsPhEG was found to be most tolerant to 1-ethyl-3-methylimidazolium acetate ([EMIM]OAc) in soluble 1% carboxymethylcellulose (CMC) and insoluble 1% Avicel substrates. At 35% concentration, this IL caused an increase in enzyme activity (up to 1.5-fold) with CMC. Several ILs were more enzyme inhibiting with insoluble Avicel than with soluble CMC. K

conclusionsThese results reveal new ways to design a beneficial combination of ILs and enzymes for the hydrolysis of lignocellulose, and the strong potential of PhEG in industrial, high substrate concentrations in aqueous IL solutions.

Indexed as

CellulaseIonic LiquidsPyrococcus horikoshiiBiomassCationsCelluloseCationsCellulaseCelluloseIonic LiquidsBiomass engineeringEnzyme inhibitionEnzyme kineticsHyperthermostable endoglucanaseIonic liquids

Identifiers

PMID35763164
PMCPMC9356960
OpenAlexW4283641957

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.