Evidence map›Paper›PMID 35763120›Full record

ArticleTransgenic research2022

The heterologous expression of conserved Glycine max (soybean) mitogen activated protein kinase 3 (MAPK3) paralogs suppresses Meloidogyne incognita parasitism in Gossypium hirsutum (upland cotton).

Vincent P Klink, Nadim W Alkharouf, Kathy S Lawrence, Bisho R Lawaju, Keshav Sharma, Prakash M Niraula, Brant T McNeece

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Transgenic research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.6field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Combating Root-Knot Nematodes (Plants (Basel, Switzerland) · 2025
    Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 7 institutions in 1 country.

Vincent P KlinkUSDA ARS NEA BARC Molecular Plant Pathology Laboratory, Building 004 Room 122 BARC-West, 10300 Baltimore Ave., Beltsville, MD, 20705, USA. vincent.klink@usda.gov.ORCID 0000-0002-7167-4032
Nadim W AlkharoufDepartment of Computer and Information Sciences, Towson University, Towson, MD, 21252, USA.ORCID 0000-0001-6038-0410
Kathy S LawrenceDepartment of Entomology and Plant Pathology, Auburn University, 209 Life Science Building, Auburn, AL, 36849, USA.ORCID 0000-0002-5358-2011
Bisho R LawajuDepartment of Entomology and Plant Pathology, Auburn University, 209 Life Science Building, Auburn, AL, 36849, USA.ORCID 0000-0001-8563-1036
Keshav SharmaDepartment of Biological Sciences, Mississippi State University, Mississippi State, MS, 39762, USA.ORCID 0000-0002-1137-5663
Prakash M NiraulaDepartment of Biological Sciences, Mississippi State University, Mississippi State, MS, 39762, USA.ORCID 0000-0002-3145-6364
Brant T McNeeceDepartment of Biological Sciences, Mississippi State University, Mississippi State, MS, 39762, USA.ORCID 0000-0002-0806-8232
Agricultural Research Service · USAuburn University · USCereal Disease Laboratory · USDelaware State University · USNorth Dakota State University · USTowson University · USWinrock International (United States) · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Two conserved Glycine max (soybean) mitogen activated protein kinase 3 (MAPK3) paralogs function in defense to the parasitic soybean cyst nematode Heterodera glycines. Gene Ontology analyses of RNA seq data obtained from MAPK3-1-overexpressing (OE) and MAPK3-2-OE roots compared to their control, as well as MAPK3-1-RNA interference (RNAi) and MAPK3-2-RNAi compared to their control, hierarchically orders the induced and suppressed genes, strengthening the hypothesis that their heterologous expression in Gossypium hirsutum (upland cotton) would impair parasitism by the root knot nematode (RKN) Meloidogyne incognita. MAPK3-1 expression (E) in G. hirsutum suppresses the production of M. incognita root galls, egg masses, and second stage juveniles (J2s) by 80.32%, 82.37%, and 88.21%, respectfully. Unexpectedly, egg number increases by 28.99% but J2s are inviable. MAPK3-2-E effects are identical, statistically. MAPK3-1-E and MAPK3-2-E decreases root mass 1.49-fold and 1.55-fold, respectively, as compared to the pRAP15-ccdB-E control. The reproductive factor (RF) of M. incognita for G. hirsutum roots expressing MAPK3-1-E or MAPK3-2-E decreases 60.39% and 50.46%, respectively, compared to controls. The results are consistent with upstream pathogen activated molecular pattern (PAMP) triggered immunity (PTI) and effector triggered immunity (ETI) functioning in defense to H. glycines. The experiments showcase the feasibility of employing MAPK3, through heterologous expression, to combat M. incognita parasitism, possibly overcoming impediments otherwise making G. hirsutum's defense platform deficient. MAPK homologs are identified in other important crop species for future functional analyses.

Indexed as

TylenchoideaAnimalsGlycine maxGossypiumMitogen-Activated Protein Kinase 3Pathogen-Associated Molecular Pattern MoleculesPlant DiseasesMitogen-Activated Protein Kinase 3Pathogen-Associated Molecular Pattern MoleculesCottonEffector triggered immunity (ETI) pathogen associated molecular pattern (PAMP) triggered immunity (PTI)Gene OntologyGlycine maxGossypium hirsutumMitogen activated protein kinase (MAPK)OverexpressionPlant parasitic nematodeRNA interference (RNAi)Soybean

Identifiers

PMID35763120
PMCPMC9489592
OpenAlexW4283645517

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.