Evidence map›Paper›PMID 35762790›Full record

ArticleApplied and environmental microbiology2022

Analysis of the Plasmid-Based

Zhili Yang, Zengping Zhang, Jiaqi Zhu, Yunhao Ma, Jianxin Wang, Jianhua Liu

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Applied and environmental microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.1field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Analysis of the Plasmid-BasedMicrobiology spectrum · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Zhili Yang *Systems Biology Laboratory, School of Marine Science and Technology, Zhejiang Ocean Universitygrid.443668.b, Zhoushan, Zhejiang, China.ORCID 0000-0002-0885-4575
Zengping Zhang *Systems Biology Laboratory, School of Marine Science and Technology, Zhejiang Ocean Universitygrid.443668.b, Zhoushan, Zhejiang, China.
Jiaqi Zhu *R&D Department, New Horizon Health Technology Company Ltd., Hangzhou, Zhejiang, China.
Yunhao MaSystems Biology Laboratory, School of Marine Science and Technology, Zhejiang Ocean Universitygrid.443668.b, Zhoushan, Zhejiang, China.
Jianxin WangSystems Biology Laboratory, School of Marine Science and Technology, Zhejiang Ocean Universitygrid.443668.b, Zhoushan, Zhejiang, China.
Jianhua LiuSystems Biology Laboratory, School of Marine Science and Technology, Zhejiang Ocean Universitygrid.443668.b, Zhoushan, Zhejiang, China.ORCID 0000-0003-1165-9348
Zhejiang Ocean University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Over 300 essential genes are predicted using transposon sequencing in the genome of Pseudomonas aeruginosa. However, methods for reverse genetic analysis of essential genes are scarce. To address this issue, we developed a three-step protocol consisting of integration of deletion plasmid, introduction of temperature-sensitive rescue plasmid, and excision of integrated-deletion plasmid to construct the plasmid-based temperature-sensitive allele of essential genes. Using

Indexed as

LipopolysaccharidesPseudomonas aeruginosaAllelesEscherichia coliO AntigensPhosphoric Monoester HydrolasesPlasmidsLipopolysaccharidesO AntigensPhosphoric Monoester Hydrolasesconditional lethal alleleessential geneslipopolysaccharide (LPS)Pseudomonas aeruginosasuicide plasmidsuppressor

Identifiers

PMID35762790
PMCPMC9317947
OpenAlexW4283696471

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.