ArticleeLife2022
CAMSAP2 organizes a γ-tubulin-independent microtubule nucleation centre through phase separation.
Article in eLife, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 39 citations in OpenAlex.
- Article
- Assembly of Microtubule Tactoids Through Condensates of PRC1 Constructs.Biomolecules · 2026Article
- The γ-Tubulin Ring Complex promotes mitotic spindle integrity and acts as a microtubule minus-end cap during mitosis.bioRxiv : the preprint server for biology · 2026Article
- Article
- Biomolecular phase separation in tumorigenesis: from aberrant condensates to therapeutic vulnerabilities.Molecular cancer · 2025Review
- Albendazole specifically disrupts microtubules and protein turnover in the tegument of the cestode Mesocestoides corti.PLoS pathogens · 2025Article
- Phase separation of microtubule-binding proteins - implications for neuronal function and disease.Journal of cell science · 2024Review
- Spermatocytes have the capacity to segregate chromosomes despite centriole duplication failure.EMBO reports · 2024Article
- The kinesin-3 KIF1C undergoes liquid-liquid phase separation for accumulation of specific transcripts at the cell periphery.The EMBO journal · 2024Article
- γ-TuRC asymmetry induces local protofilament mismatch at the RanGTP-stimulated microtubule minus end.The EMBO journal · 2024Article
- Biomolecular Condensates Decipher Molecular Codes of Cell Fate: From Biophysical Fundamentals to Therapeutic Practices.International journal of molecular sciences · 2024Review
- CAMSAPs and nucleation-promoting factors control microtubule release from γ-TuRC.Nature cell biology · 2024Article
- Patronin regulates presynaptic microtubule organization and neuromuscular junction development iniScience · 2024Article
- Multifaceted modes of γ-tubulin complex recruitment and microtubule nucleation at mitotic centrosomes.The Journal of cell biology · 2023Article
- [High expression of CAMSAP2 promotes invasion and metastasis of gastric cancer cells by upregulating TGF-β signaling].Nan fang yi ke da xue xue bao = Journal of Southern Medical University · 2023Article
- Mechanisms underlying spindle assembly and robustness.Nature reviews. Molecular cell biology · 2023Review
- Interplay of self-organization of microtubule asters and crosslinking protein condensates.PNAS nexus · 2023Article
- Preference of CAMSAP3 for expanded microtubule lattice contributes to stabilization of the minus end.Life science alliance · 2023Article
- Morphological Evidence for Novel Roles of Microtubules in Macrophage Phagocytosis.International journal of molecular sciences · 2023Article
- Microtubule-associated proteins promote microtubule generation in the absence of γ-tubulin in human colon cancer cells.The Journal of cell biology · 2021Article
Corrections and comments
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Authors and funding
20 authors at 6 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microtubules are dynamic polymers consisting of αβ-tubulin heterodimers. The initial polymerization process, called microtubule nucleation, occurs spontaneously via αβ-tubulin. Since a large energy barrier prevents microtubule nucleation in cells, the γ-tubulin ring complex is recruited to the centrosome to overcome the nucleation barrier. However, a considerable number of microtubules can polymerize independently of the centrosome in various cell types. Here, we present evidence that the minus-end-binding calmodulin-regulated spectrin-associated protein 2 (CAMSAP2) serves as a strong nucleator for microtubule formation by significantly reducing the nucleation barrier. CAMSAP2 co-condensates with αβ-tubulin via a phase separation process, producing plenty of nucleation intermediates. Microtubules then radiate from the co-condensates, resulting in aster-like structure formation. CAMSAP2 localizes at the co-condensates and decorates the radiating microtubule lattices to some extent. Taken together, these in vitro findings suggest that CAMSAP2 supports microtubule nucleation and growth by organizing a nucleation centre as well as by stabilizing microtubule intermediates and growing microtubules.
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