Evidence map›Paper›PMID 35761332›Full record

ArticleJournal of nanobiotechnology2022

Delivery of engineered extracellular vesicles with miR-29b editing system for muscle atrophy therapy.

Rui Chen, Weilin Yuan, Yongjun Zheng, Xiaolan Zhu, Bing Jin, Tingting Yang, Yuwei Yan, Wanru Xu, Hongjian Chen, Juan Gao and 5 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of nanobiotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
2.0field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 25 citations in OpenAlex.

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  13. Extracellular Vesicles: A New Star for Gene Drug Delivery.International journal of nanomedicine · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 7 institutions in 3 countries.

Rui Chen *Institute of Geriatrics, The Sixth People's Hospital of Nantong), School of Medicine, Shanghai University, Affiliated Nantong Hospital of Shanghai University, Shanghai University, Nantong, 226011, China.
Weilin Yuan *Institute of Geriatrics, The Sixth People's Hospital of Nantong), School of Medicine, Shanghai University, Affiliated Nantong Hospital of Shanghai University, Shanghai University, Nantong, 226011, China.
Yongjun Zheng *Division of Pain Management, Huadong Hospital Affiliated to Fudan University, Shanghai, 200040, China.
Xiaolan ZhuInstitute of Geriatrics, The Sixth People's Hospital of Nantong), School of Medicine, Shanghai University, Affiliated Nantong Hospital of Shanghai University, Shanghai University, Nantong, 226011, China.
Bing JinInstitute of Geriatrics, The Sixth People's Hospital of Nantong), School of Medicine, Shanghai University, Affiliated Nantong Hospital of Shanghai University, Shanghai University, Nantong, 226011, China.
Tingting YangInstitute of Geriatrics, The Sixth People's Hospital of Nantong), School of Medicine, Shanghai University, Affiliated Nantong Hospital of Shanghai University, Shanghai University, Nantong, 226011, China.
Yuwei YanInstitute of Geriatrics, The Sixth People's Hospital of Nantong), School of Medicine, Shanghai University, Affiliated Nantong Hospital of Shanghai University, Shanghai University, Nantong, 226011, China.
Wanru XuInstitute of Geriatrics, The Sixth People's Hospital of Nantong), School of Medicine, Shanghai University, Affiliated Nantong Hospital of Shanghai University, Shanghai University, Nantong, 226011, China.
Hongjian ChenInstitute of Geriatrics, The Sixth People's Hospital of Nantong), School of Medicine, Shanghai University, Affiliated Nantong Hospital of Shanghai University, Shanghai University, Nantong, 226011, China.
Juan GaoInstitute of Geriatrics, The Sixth People's Hospital of Nantong), School of Medicine, Shanghai University, Affiliated Nantong Hospital of Shanghai University, Shanghai University, Nantong, 226011, China.
Guoping LiCardiovascular Division of the Massachusetts General Hospital, Harvard Medical School, Boston, MA, 02114, USA.
Priyanka GokulnathCardiovascular Division of the Massachusetts General Hospital, Harvard Medical School, Boston, MA, 02114, USA.
Gururaja VulugundamInstitute of Biochemistry and Cellular Biology, National Research Council of Italy, Napoli, 80131, Italy.
Jin LiInstitute of Geriatrics, The Sixth People's Hospital of Nantong), School of Medicine, Shanghai University, Affiliated Nantong Hospital of Shanghai University, Shanghai University, Nantong, 226011, China. sszxlijin@163.com.
Junjie XiaoInstitute of Geriatrics, The Sixth People's Hospital of Nantong), School of Medicine, Shanghai University, Affiliated Nantong Hospital of Shanghai University, Shanghai University, Nantong, 226011, China. junjiexiao@shu.edu.cn.
Affiliated Hospital of Nantong University · CNShanghai University · CNNantong University · CNHarvard University · USHuadong Hospital · CNMassachusetts General Hospital · USNational Research Council · IT

Funding

Innovation Program of Shanghai Municipal Education Commission 2017-01-07-00-09-E00042National Key R&D Program of China 2020YFA0803800National Natural Science Foundation of China 81900359National Natural Science Foundation of China 82020108002 and 81911540486Shanghai Sailing Program 19YF1416400the "Chen Guang" project supported by the Shanghai Municipal Education Commission and Shanghai Education Development Foundation 19CG45the "Dawn" Program of Shanghai Education Commission 19SG34the grant from Science and Technology Commission of Shanghai Municipality 20DZ2255400 and 21XD1421300the grant from Science and Technology Commission of Shanghai Municipality 22010500200
6 · The paper itself

Abstract

Muscle atrophy is a frequently observed complication, characterized by the loss of muscle mass and strength, which diminishes the quality of life and survival. No effective therapy except exercise is currently available. In our previous study, repressing miR-29b has been shown to reduce muscle atrophy. In our current study, we have constructed artificially engineered extracellular vesicles for the delivery of CRISPR/Cas9 to target miR-29b (EVs-Cas9-29b). EVs-Cas9-29b has shown a favorable functional effect with respect to miR-29b repression in a specific and rapid manner by gene editing. In in vitro conditions, EVs-Cas9-29b could protect against muscle atrophy induced by dexamethasone (Dex), angiotensin II (AngII), and tumor necrosis factor-alpha (TNF-α). And EVs-Cas9-29b introduced in vivo preserved muscle function in the well-established immobilization and denervation-induced muscle atrophy mice model. Our work demonstrates an engineered extracellular vesicles delivery of the miR-29b editing system, which could be potentially used for muscle atrophy therapy.

Indexed as

Extracellular VesiclesMicroRNAsMuscular AtrophyAnimalsCRISPR-Cas SystemsMiceTumor Necrosis Factor-alphaMicroRNAsMIRN29 microRNA, mouseTumor Necrosis Factor-alphaCRISPR/Cas9Engineered extracellular vesiclesmiR-29bMuscle atrophyTherapy

Identifiers

PMID35761332
PMCPMC9235146
OpenAlexW4283690207

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.