ArticleMethods in molecular biology (Clifton, N.J.)2022
Detection of Gasdermin Activation and Lytic Cell Death During Pyroptosis and Apoptosis.
Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed, 6 citations in OpenAlex.
- Effects and mechanisms of Salmonella plasmid virulence gene spv on host-regulated cell death.Current microbiology · 2024Review
- Optogenetic Induction of Pyroptosis, Necroptosis, and Apoptosis in Mammalian Cell Lines.Bio-protocol · 2023Article
- Gasdermins and pyroptosis in the kidney.Nature reviews. Nephrology · 2023Review
- Regulated necrosis role in inflammation and repair in acute kidney injury.Frontiers in immunology · 2023Review
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Authors and funding
3 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cytosolic pattern recognition receptors trigger pyroptosis by detection of danger- or pathogen-associated molecular patterns. These receptors initiate the assembly of inflammasomes, multimeric protein complexes that drive caspase-1 activation. Active caspase-1 cleaves the proinflammatory cytokines IL-1β and IL-18 and the pore-forming protein gasdermin-D (GSDMD) thereby liberating its N-terminal domain. The GSDMD N-termini form multimeric pores at the plasma membrane that allow leakage of intracellular content and ultimately trigger a type of cell death called "pyroptosis." Emerging studies have revealed that GSDMD is also processed by apoptotic caspases-8/-3/-7. In this chapter, we aim to describe methods to monitor lytic cell death and to distinguish between GSDMD processing events and the GSDMD fragments that are generated after pyroptosis or apoptosis induction. We also illustrate the difference between GSDMD pore formation, and final cell lysis, and how this affects to the release of intracellular content. Finally, we show that the activation of another pore-forming protein, gasdermin-E, does not exclusively translate into lytic cell death in bone marrow-derived macrophages.
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Registered trials
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