Evidence map›Paper›PMID 35759174›Full record

ArticleInfection2023

Implementing the Lolli-Method and pooled RT-qPCR testing for SARS-CoV-2 surveillance in schools: a pilot project.

Alina Chloé Kretschmer, Lena Junker, Felix Dewald, Viktoria Linne, Lea Hennen, Gibran Horemheb-Rubio, Rolf Kaiser, Gertrud Steger, Alexander Joachim, Jana Schönenkorb and 18 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Infection, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.6field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 6 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

28 authors at 4 institutions in 1 country.

Alina Chloé KretschmerDepartment I of Internal Medicine, Division of Infectious Diseases, University Hospital Cologne, University of Cologne, Kerpener Str. 62, 50937, Cologne, Germany.
Lena JunkerDepartment I of Internal Medicine, Division of Infectious Diseases, University Hospital Cologne, University of Cologne, Kerpener Str. 62, 50937, Cologne, Germany.
Felix DewaldCenter for Molecular Medicine Cologne, University of Cologne, 50931, Cologne, Germany.
Viktoria LinneDepartment I of Internal Medicine, Division of Infectious Diseases, University Hospital Cologne, University of Cologne, Kerpener Str. 62, 50937, Cologne, Germany.
Lea HennenDepartment I of Internal Medicine, Division of Infectious Diseases, University Hospital Cologne, University of Cologne, Kerpener Str. 62, 50937, Cologne, Germany.
Gibran Horemheb-RubioInstitute of Virology, University Hospital Cologne, University of Cologne, Cologne, Germany.
Rolf KaiserInstitute of Virology, University Hospital Cologne, University of Cologne, Cologne, Germany.
Gertrud StegerInstitute of Virology, University Hospital Cologne, University of Cologne, Cologne, Germany.
Alexander JoachimDepartment of Pediatrics, University Hospital Cologne, University of Cologne, Cologne, Germany.
Jana SchönenkorbDepartment of Pediatrics, University Hospital Cologne, University of Cologne, Cologne, Germany.
Zülfü Cem CosgunDepartment of Pediatrics, University Hospital Cologne, University of Cologne, Cologne, Germany.
Neslihan MühlhansInstitute of Virology, University Hospital Cologne, University of Cologne, Cologne, Germany.
Eva HegerInstitute of Virology, University Hospital Cologne, University of Cologne, Cologne, Germany.
Elena KnopsInstitute of Virology, University Hospital Cologne, University of Cologne, Cologne, Germany.
Charlotte LeisseDepartment I of Internal Medicine, Division of Infectious Diseases, University Hospital Cologne, University of Cologne, Kerpener Str. 62, 50937, Cologne, Germany.
Barbora KesselDepartment of Epidemiology, Helmholtz Centre for Infection Research, Brunswick, Germany.
Torben HeinsohnDepartment of Epidemiology, Helmholtz Centre for Infection Research, Brunswick, Germany.
Isti RodiahDepartment of Epidemiology, Helmholtz Centre for Infection Research, Brunswick, Germany.
Berit LangeDepartment of Epidemiology, Helmholtz Centre for Infection Research, Brunswick, Germany.
Anne Lena RitterSchool Department Cologne, Cologne, Germany.
Mira FriesDepartment I of Internal Medicine, Division of Infectious Diseases, University Hospital Cologne, University of Cologne, Kerpener Str. 62, 50937, Cologne, Germany.
Annelene KossowPublic Health Department Cologne, Cologne, Germany.
Johannes NießenPublic Health Department Cologne, Cologne, Germany.
Jörg DötschDepartment of Pediatrics, University Hospital Cologne, University of Cologne, Cologne, Germany.
Florian KleinGerman Center for Infection Research (DZIF), Partner Site Bonn-Cologne, Cologne, Germany.
Jan RybnikerDepartment I of Internal Medicine, Division of Infectious Diseases, University Hospital Cologne, University of Cologne, Kerpener Str. 62, 50937, Cologne, Germany.
Gerd FätkenheuerDepartment I of Internal Medicine, Division of Infectious Diseases, University Hospital Cologne, University of Cologne, Kerpener Str. 62, 50937, Cologne, Germany.
Isabelle SuárezDepartment I of Internal Medicine, Division of Infectious Diseases, University Hospital Cologne, University of Cologne, Kerpener Str. 62, 50937, Cologne, Germany. isabelle.suarez@uk-koeln.de.ORCID http://orcid.org/0000-0002-9863-9675
University of Cologne · DECBS International Business School · DEHelmholtz Centre for Infection Research · DEGerman Center for Infection Research · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeSchool closures have been used as part of lockdown strategies to contain the spread of SARS-CoV-2, adversely affecting children's health and education. To ensure the accessibility of educational institutions without exposing society to the risk of increased transmissions, it is essential to establish SARS-CoV-2 testing strategies that are child-friendly, scalable and implementable in a daily school routine. Self-sampling using non-invasive saliva swabs combined with pooled RT-qPCR testing (Lolli-Method) has been proven to be a sensitive method for the detection of SARS-CoV-2.

methodsWe conducted a pilot project in Cologne, Germany, designed to determine the feasibility of a large-scale rollout of the Lolli-Method for testing without any additional on-site medical staff in schools. Over a period of three weeks, students from 22 schools were sampled using the Lolli-Method. At the end of the project, teachers were asked to evaluate the overall acceptance of the project.

resultsWe analyzed a total of 757 pooled RT-qPCRs obtained from 8,287 individual swabs and detected 7 SARS-CoV-2 infected individuals. The Lolli-Method was shown to be a feasible and accepted testing strategy whose application is only slightly disruptive to the daily school routine.

conclusionOur observations suggest that the Lolli-Method in combination with pooled RT-qPCR can be implemented for SARS-CoV-2 surveillance in daily school routine, applicable on a large scale.

Indexed as

COVID-19SARS-CoV-2Communicable Disease ControlCOVID-19 TestingHumansPilot ProjectsSchoolsCOVID-19Lolli-MethodPooled testingRT-qPCRSARS-CoV-2School

Identifiers

PMID35759174
PMCPMC9243733
OpenAlexW4283660159

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.